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primary human bone-marrow cd34 + cells  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc primary human bone-marrow cd34 + cells
    ( A ) Clustering of the DEGs during transdifferentiation between dCas9-DNMT3A CTRL and IL1RN edited cells. ( n = 2 biologically independent replicates, FDR < 0.05). TC1 to TC4, transdifferentiation clusters. Representative genes for each cluster are annotated. ( B ) Gene Ontology Biological Processes (GO:BP) enrichment analysis for the genes associated with clusters in (A). The top five most significant terms for each cluster are plotted. Significant terms ( q value < 0.05) are highlighted with a black stroke. ( C ) Mean fluorescence intensity (MFI) of macrophage-lineage surface markers in CTRL and edited iMacs. ( D ) Representative histograms showing signal intensity for selected cell surface markers in CTRL and edited iMacs. ( E ) Phagocytic capacity evaluation [by fluorescence-activated cell sorting (FACS)] for CTRL and edited iMacs. Left, histogram showing the uptake of blue fluorescent beads. Right, blue beads MFI quantification. Unpaired two-tailed Student’s t test, n = 3, means ± SEM, (*** P < 0.001). ( F ) Up, schematic of the IL1RN shRNAs experiments during transdifferentiation. Bottom, MFI quantification of myeloid markers in shLuc and shIL1RN iMacs. Two-way ANOVA with Dunnett’s post hoc test, n = 3, means ± SEM, (**** P < 0.0001). ( G ) Phagocytic capacity evaluation (by FACS) for shLuc and shIL1RN iMacs. Left, histogram showing the uptake of blue fluorescent beads. Right, blue beads MFI quantification. Two-way ANOVA with Dunnett’s post hoc test, n = 3, means ± SEM, (**** P < 0.0001). ( H ) Left, schematic of the colony-forming assay performed in shLuc and shIL1RN bone marrow–derived human <t>CD34</t> + cells; CFU, <t>colony</t> <t>forming</t> <t>unit.</t> Right, total number of CFUs obtained from shLuc and shIL1RN_1/_2 CD34 + cells. n = 3 biologically independent samples per group, means ± SEM. ( I ) Comparison of different types of CFUs obtained from shLuc and shIL1RN_1/_2 CD34 + cells. Two-way ANOVA with Dunnett’s post hoc test, n = 3, means ± SEM,(* P < 0.05; ** P < 0.01; **** P < 0.0001).
    Primary Human Bone Marrow Cd34 + Cells, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+human+bone-marrow+cd34+++cells/easysep+human+t+cell+isolation+kit/pmc12266127-309-0-6
    Average 90 stars, based on 1 article reviews
    primary human bone-marrow cd34 + cells - by Bioz Stars, 2026-11
    90/100 stars

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    1) Product Images from "Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing"

    Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing

    Journal: Science Advances

    doi: 10.1126/sciadv.adt1644

    ( A ) Clustering of the DEGs during transdifferentiation between dCas9-DNMT3A CTRL and IL1RN edited cells. ( n = 2 biologically independent replicates, FDR < 0.05). TC1 to TC4, transdifferentiation clusters. Representative genes for each cluster are annotated. ( B ) Gene Ontology Biological Processes (GO:BP) enrichment analysis for the genes associated with clusters in (A). The top five most significant terms for each cluster are plotted. Significant terms ( q value < 0.05) are highlighted with a black stroke. ( C ) Mean fluorescence intensity (MFI) of macrophage-lineage surface markers in CTRL and edited iMacs. ( D ) Representative histograms showing signal intensity for selected cell surface markers in CTRL and edited iMacs. ( E ) Phagocytic capacity evaluation [by fluorescence-activated cell sorting (FACS)] for CTRL and edited iMacs. Left, histogram showing the uptake of blue fluorescent beads. Right, blue beads MFI quantification. Unpaired two-tailed Student’s t test, n = 3, means ± SEM, (*** P < 0.001). ( F ) Up, schematic of the IL1RN shRNAs experiments during transdifferentiation. Bottom, MFI quantification of myeloid markers in shLuc and shIL1RN iMacs. Two-way ANOVA with Dunnett’s post hoc test, n = 3, means ± SEM, (**** P < 0.0001). ( G ) Phagocytic capacity evaluation (by FACS) for shLuc and shIL1RN iMacs. Left, histogram showing the uptake of blue fluorescent beads. Right, blue beads MFI quantification. Two-way ANOVA with Dunnett’s post hoc test, n = 3, means ± SEM, (**** P < 0.0001). ( H ) Left, schematic of the colony-forming assay performed in shLuc and shIL1RN bone marrow–derived human CD34 + cells; CFU, colony forming unit. Right, total number of CFUs obtained from shLuc and shIL1RN_1/_2 CD34 + cells. n = 3 biologically independent samples per group, means ± SEM. ( I ) Comparison of different types of CFUs obtained from shLuc and shIL1RN_1/_2 CD34 + cells. Two-way ANOVA with Dunnett’s post hoc test, n = 3, means ± SEM,(* P < 0.05; ** P < 0.01; **** P < 0.0001).
    Figure Legend Snippet: ( A ) Clustering of the DEGs during transdifferentiation between dCas9-DNMT3A CTRL and IL1RN edited cells. ( n = 2 biologically independent replicates, FDR < 0.05). TC1 to TC4, transdifferentiation clusters. Representative genes for each cluster are annotated. ( B ) Gene Ontology Biological Processes (GO:BP) enrichment analysis for the genes associated with clusters in (A). The top five most significant terms for each cluster are plotted. Significant terms ( q value < 0.05) are highlighted with a black stroke. ( C ) Mean fluorescence intensity (MFI) of macrophage-lineage surface markers in CTRL and edited iMacs. ( D ) Representative histograms showing signal intensity for selected cell surface markers in CTRL and edited iMacs. ( E ) Phagocytic capacity evaluation [by fluorescence-activated cell sorting (FACS)] for CTRL and edited iMacs. Left, histogram showing the uptake of blue fluorescent beads. Right, blue beads MFI quantification. Unpaired two-tailed Student’s t test, n = 3, means ± SEM, (*** P < 0.001). ( F ) Up, schematic of the IL1RN shRNAs experiments during transdifferentiation. Bottom, MFI quantification of myeloid markers in shLuc and shIL1RN iMacs. Two-way ANOVA with Dunnett’s post hoc test, n = 3, means ± SEM, (**** P < 0.0001). ( G ) Phagocytic capacity evaluation (by FACS) for shLuc and shIL1RN iMacs. Left, histogram showing the uptake of blue fluorescent beads. Right, blue beads MFI quantification. Two-way ANOVA with Dunnett’s post hoc test, n = 3, means ± SEM, (**** P < 0.0001). ( H ) Left, schematic of the colony-forming assay performed in shLuc and shIL1RN bone marrow–derived human CD34 + cells; CFU, colony forming unit. Right, total number of CFUs obtained from shLuc and shIL1RN_1/_2 CD34 + cells. n = 3 biologically independent samples per group, means ± SEM. ( I ) Comparison of different types of CFUs obtained from shLuc and shIL1RN_1/_2 CD34 + cells. Two-way ANOVA with Dunnett’s post hoc test, n = 3, means ± SEM,(* P < 0.05; ** P < 0.01; **** P < 0.0001).

    Techniques Used: Fluorescence, FACS, Two Tailed Test, Derivative Assay, Comparison

    Related Articles

    Cell Culture:

    Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
    Article Snippet: Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions.

    Article Title: Cancer antigen targets and uses thereof
    Article Snippet: Primary human bone marrow CD34+ cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Flow-cytometric analysis The following antibodies were used to define antigen expression by flow-cytometry: CD82-PE (Biolegend, 342103); CD120b (TNF-RII)-PE (Miltenyi, 130–107-740); EMR2-FITC, -APC (Miltenyi, 130–104-654, 130–104-656); ITGB5-PE (Biolegend, 345203); CCR1-PE, -APC (Miltenyi, 130–100-367, 130–100-358); CD96-APC (Miltenyi, 130–101-031); PTPRJ (CD148)-PE (life technologies, A15799); CD70-PE, -FITC (Biolegend, 355104, 355106); CD85d (ILT4)-PE -APC (Miltenyi, 130–100-567, 130–100-559); LTB4R1-AF700 (Novus Biologicals, FAB099N); CD85h (ILT1)-APC (Miltenyi, 130–100-920); TLR2-APC (Miltenyi, 130–099-020); CR1 (aka CD35)-APC (Miltenyi, 130–099-923); ITGAX (CD11c)-APC (Biolegend, 301613); EMB (abcam, 179801); EMC10 (abcam PA5–25112); LILRB3-PE (Miltenyi, 130–101-662); LILRB4-APC (R&D, FAB24251A); DAGLB (abcam, PA5–26331); P2RY13 (Novus Biologicals, NBP2–37382); LILRA6-APC (Miltenyi, 130–101-665); SLC30A1 (Alomone labs, AZT-011); SLC6A6 (LSBio, LS-C179237); SEMA4A (R&D, FAB4694A); CD123-PE (BD Biosciences, 555644); CLEC12A-PE (Miltenyi, 130–106-482); CD33-APC (Miltenyi, 130–098-864); CD38-BV421 (BD Biosciences, 562444); CD34-PE/Cy7 (Biolegend, 343515); CD45RA-BV640 (Biolegend, 304135); CD90-FITC (BD Biosciences, 555595).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Primary human bone marrow CD34 + cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: RNA sequestration in P-bodies sustains myeloid leukaemia.
    Article Snippet: Srikanth Kodali , Ludovica Proietti , Gemma Valcarcel , Anna V. López-Rubio , Patrizia Pessina , Thomas Eder , Junchao Shi , Annie Jen , Núria Lupión-Garcia , Anne C. Starner, Mason D. Bartels, Yingzhi Cui , Caroline M. Sands , Ainoa Planas-Riverola, Alba Martínez , Talia Velasco-Hernandez , Laureano Tomás-Daza , Bernhard Alber , Gabriele Manhart, Isabella Maria Mayer , Karoline Kollmann , Alessandro Fatica , Pablo Menendez, Evgenia Shishkova, Rachel E. Rau, Biola M. Javierre , Joshua Coon , Qi Chen , Eric L. Van Nostrand , Jose L. Sardina , Florian Grebien & Bruno Di Stefano

    Article Title: Erythrocyte CD55 mediates the internalization of Plasmodium falciparum parasites
    Article Snippet: Bone-marrow-derived primary human CD34 +HSPCs (Stem Cell Technologies) were cultured in erythroid differentiation medium (cPIMDM) composed of Iscove Basal Medium (IMDM) (Biochrom) supplemented with 4 mM L-Glutamine (Sigma), 330 μg/ml holo-transferrin (BBI Solutions), 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix), 10 −6 M hydrocortisone (Sigma), 100 ng/ml SCF (R and D Systems), 5 ng/ml IL-3 (R and D Systems), 3 IU/ml Epo (Amgen) and 5% plasma (Octapharma) at 37°C in 5% CO 2 in air, as previously described ( ; ).

    Article Title: AMPK-PERK axis represses oxidative metabolism and enhances apoptotic priming of mitochondria in acute myeloid leukemia.
    Article Snippet: 2015-08-11-DC Primary Human bone marrow CD34+ normal hematopoietic cells StemCell Technologies 70002.2 Chemicals, peptides, and recombinant proteins AMPK activator GSK621 Selleck chemicals S7898, CAS 1346607-05-3 AMPK activator MK8722 Aobious AOB33226, CAS 1394371-71-1 AMPK activator PF739 Aobious AOB33584, CAS 1852452-14-2 ER stress inducer Thapsigargin Sigma-Aldrich T9033, CAS 67526-95-8 AMPK inhibitor Compound C Selleck chemicals S7306, CAS 1219168-18-9 Bcl2 inhibitor Venetoclax Selleck chemicals S8048, CAS 1257044-40-8 6-Bn-ATP-g-S Biolog Life Science Institute B072, CAS 944834-42-8 p- Nitrobenzyl Mesylate Abcam ab138910, CAS 39628-94-9 GST-tagged human recombinant PERK Thermo-Fischer Scientifc PV5106 GST-tagged human recombinant ULK-1 Thermo-Fischer Scientifc PV6430 GST-tagged human recombinant RSK-2 Thermo-Fischer Scientifc PV3323 PUMA2A New England Peptide EQWAREIGAQARRMAADLNA BAD New England Peptide LWAAQRYGRELRRMSDEFEGSFKGL W-HRK New England Peptide WSSAAQLTAARLKALGDELHQ BIM New England Peptide MRPEIWIAQELRRIGDEFNA PUMA New England Peptide EQWAREIGAQLRRMADDLNA NOXAA New England Peptide AELPPEFAAQLRKIGDKVYC (Continued on next page) e2 Cell Reports 38, 110197, January 4, 2022

    Article Title: RNA sequestration in P-bodies sustains myeloid leukemia
    Article Snippet: Primary human bone marrow CD34 + cells (STEMCELL Technologies cat. no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies cat. no 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies cat. no 2690).

    shRNA:

    Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
    Article Snippet: Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions.

    Article Title: Cancer antigen targets and uses thereof
    Article Snippet: Primary human bone marrow CD34+ cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Flow-cytometric analysis The following antibodies were used to define antigen expression by flow-cytometry: CD82-PE (Biolegend, 342103); CD120b (TNF-RII)-PE (Miltenyi, 130–107-740); EMR2-FITC, -APC (Miltenyi, 130–104-654, 130–104-656); ITGB5-PE (Biolegend, 345203); CCR1-PE, -APC (Miltenyi, 130–100-367, 130–100-358); CD96-APC (Miltenyi, 130–101-031); PTPRJ (CD148)-PE (life technologies, A15799); CD70-PE, -FITC (Biolegend, 355104, 355106); CD85d (ILT4)-PE -APC (Miltenyi, 130–100-567, 130–100-559); LTB4R1-AF700 (Novus Biologicals, FAB099N); CD85h (ILT1)-APC (Miltenyi, 130–100-920); TLR2-APC (Miltenyi, 130–099-020); CR1 (aka CD35)-APC (Miltenyi, 130–099-923); ITGAX (CD11c)-APC (Biolegend, 301613); EMB (abcam, 179801); EMC10 (abcam PA5–25112); LILRB3-PE (Miltenyi, 130–101-662); LILRB4-APC (R&D, FAB24251A); DAGLB (abcam, PA5–26331); P2RY13 (Novus Biologicals, NBP2–37382); LILRA6-APC (Miltenyi, 130–101-665); SLC30A1 (Alomone labs, AZT-011); SLC6A6 (LSBio, LS-C179237); SEMA4A (R&D, FAB4694A); CD123-PE (BD Biosciences, 555644); CLEC12A-PE (Miltenyi, 130–106-482); CD33-APC (Miltenyi, 130–098-864); CD38-BV421 (BD Biosciences, 562444); CD34-PE/Cy7 (Biolegend, 343515); CD45RA-BV640 (Biolegend, 304135); CD90-FITC (BD Biosciences, 555595).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Primary human bone marrow CD34 + cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: RNA sequestration in P-bodies sustains myeloid leukaemia.
    Article Snippet: Srikanth Kodali , Ludovica Proietti , Gemma Valcarcel , Anna V. López-Rubio , Patrizia Pessina , Thomas Eder , Junchao Shi , Annie Jen , Núria Lupión-Garcia , Anne C. Starner, Mason D. Bartels, Yingzhi Cui , Caroline M. Sands , Ainoa Planas-Riverola, Alba Martínez , Talia Velasco-Hernandez , Laureano Tomás-Daza , Bernhard Alber , Gabriele Manhart, Isabella Maria Mayer , Karoline Kollmann , Alessandro Fatica , Pablo Menendez, Evgenia Shishkova, Rachel E. Rau, Biola M. Javierre , Joshua Coon , Qi Chen , Eric L. Van Nostrand , Jose L. Sardina , Florian Grebien & Bruno Di Stefano

    Article Title: Erythrocyte CD55 mediates the internalization of Plasmodium falciparum parasites
    Article Snippet: Bone-marrow-derived primary human CD34 +HSPCs (Stem Cell Technologies) were cultured in erythroid differentiation medium (cPIMDM) composed of Iscove Basal Medium (IMDM) (Biochrom) supplemented with 4 mM L-Glutamine (Sigma), 330 μg/ml holo-transferrin (BBI Solutions), 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix), 10 −6 M hydrocortisone (Sigma), 100 ng/ml SCF (R and D Systems), 5 ng/ml IL-3 (R and D Systems), 3 IU/ml Epo (Amgen) and 5% plasma (Octapharma) at 37°C in 5% CO 2 in air, as previously described ( ; ).

    Article Title: AMPK-PERK axis represses oxidative metabolism and enhances apoptotic priming of mitochondria in acute myeloid leukemia.
    Article Snippet: 2015-08-11-DC Primary Human bone marrow CD34+ normal hematopoietic cells StemCell Technologies 70002.2 Chemicals, peptides, and recombinant proteins AMPK activator GSK621 Selleck chemicals S7898, CAS 1346607-05-3 AMPK activator MK8722 Aobious AOB33226, CAS 1394371-71-1 AMPK activator PF739 Aobious AOB33584, CAS 1852452-14-2 ER stress inducer Thapsigargin Sigma-Aldrich T9033, CAS 67526-95-8 AMPK inhibitor Compound C Selleck chemicals S7306, CAS 1219168-18-9 Bcl2 inhibitor Venetoclax Selleck chemicals S8048, CAS 1257044-40-8 6-Bn-ATP-g-S Biolog Life Science Institute B072, CAS 944834-42-8 p- Nitrobenzyl Mesylate Abcam ab138910, CAS 39628-94-9 GST-tagged human recombinant PERK Thermo-Fischer Scientifc PV5106 GST-tagged human recombinant ULK-1 Thermo-Fischer Scientifc PV6430 GST-tagged human recombinant RSK-2 Thermo-Fischer Scientifc PV3323 PUMA2A New England Peptide EQWAREIGAQARRMAADLNA BAD New England Peptide LWAAQRYGRELRRMSDEFEGSFKGL W-HRK New England Peptide WSSAAQLTAARLKALGDELHQ BIM New England Peptide MRPEIWIAQELRRIGDEFNA PUMA New England Peptide EQWAREIGAQLRRMADDLNA NOXAA New England Peptide AELPPEFAAQLRKIGDKVYC (Continued on next page) e2 Cell Reports 38, 110197, January 4, 2022

    Article Title: RNA sequestration in P-bodies sustains myeloid leukemia
    Article Snippet: Primary human bone marrow CD34 + cells (STEMCELL Technologies cat. no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies cat. no 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies cat. no 2690).

    Fluorescence:

    Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
    Article Snippet: Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions.

    Article Title: Cancer antigen targets and uses thereof
    Article Snippet: Primary human bone marrow CD34+ cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Flow-cytometric analysis The following antibodies were used to define antigen expression by flow-cytometry: CD82-PE (Biolegend, 342103); CD120b (TNF-RII)-PE (Miltenyi, 130–107-740); EMR2-FITC, -APC (Miltenyi, 130–104-654, 130–104-656); ITGB5-PE (Biolegend, 345203); CCR1-PE, -APC (Miltenyi, 130–100-367, 130–100-358); CD96-APC (Miltenyi, 130–101-031); PTPRJ (CD148)-PE (life technologies, A15799); CD70-PE, -FITC (Biolegend, 355104, 355106); CD85d (ILT4)-PE -APC (Miltenyi, 130–100-567, 130–100-559); LTB4R1-AF700 (Novus Biologicals, FAB099N); CD85h (ILT1)-APC (Miltenyi, 130–100-920); TLR2-APC (Miltenyi, 130–099-020); CR1 (aka CD35)-APC (Miltenyi, 130–099-923); ITGAX (CD11c)-APC (Biolegend, 301613); EMB (abcam, 179801); EMC10 (abcam PA5–25112); LILRB3-PE (Miltenyi, 130–101-662); LILRB4-APC (R&D, FAB24251A); DAGLB (abcam, PA5–26331); P2RY13 (Novus Biologicals, NBP2–37382); LILRA6-APC (Miltenyi, 130–101-665); SLC30A1 (Alomone labs, AZT-011); SLC6A6 (LSBio, LS-C179237); SEMA4A (R&D, FAB4694A); CD123-PE (BD Biosciences, 555644); CLEC12A-PE (Miltenyi, 130–106-482); CD33-APC (Miltenyi, 130–098-864); CD38-BV421 (BD Biosciences, 562444); CD34-PE/Cy7 (Biolegend, 343515); CD45RA-BV640 (Biolegend, 304135); CD90-FITC (BD Biosciences, 555595).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Primary human bone marrow CD34 + cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: RNA sequestration in P-bodies sustains myeloid leukaemia.
    Article Snippet: Srikanth Kodali , Ludovica Proietti , Gemma Valcarcel , Anna V. López-Rubio , Patrizia Pessina , Thomas Eder , Junchao Shi , Annie Jen , Núria Lupión-Garcia , Anne C. Starner, Mason D. Bartels, Yingzhi Cui , Caroline M. Sands , Ainoa Planas-Riverola, Alba Martínez , Talia Velasco-Hernandez , Laureano Tomás-Daza , Bernhard Alber , Gabriele Manhart, Isabella Maria Mayer , Karoline Kollmann , Alessandro Fatica , Pablo Menendez, Evgenia Shishkova, Rachel E. Rau, Biola M. Javierre , Joshua Coon , Qi Chen , Eric L. Van Nostrand , Jose L. Sardina , Florian Grebien & Bruno Di Stefano

    Article Title: Erythrocyte CD55 mediates the internalization of Plasmodium falciparum parasites
    Article Snippet: Bone-marrow-derived primary human CD34 +HSPCs (Stem Cell Technologies) were cultured in erythroid differentiation medium (cPIMDM) composed of Iscove Basal Medium (IMDM) (Biochrom) supplemented with 4 mM L-Glutamine (Sigma), 330 μg/ml holo-transferrin (BBI Solutions), 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix), 10 −6 M hydrocortisone (Sigma), 100 ng/ml SCF (R and D Systems), 5 ng/ml IL-3 (R and D Systems), 3 IU/ml Epo (Amgen) and 5% plasma (Octapharma) at 37°C in 5% CO 2 in air, as previously described ( ; ).

    Article Title: AMPK-PERK axis represses oxidative metabolism and enhances apoptotic priming of mitochondria in acute myeloid leukemia.
    Article Snippet: 2015-08-11-DC Primary Human bone marrow CD34+ normal hematopoietic cells StemCell Technologies 70002.2 Chemicals, peptides, and recombinant proteins AMPK activator GSK621 Selleck chemicals S7898, CAS 1346607-05-3 AMPK activator MK8722 Aobious AOB33226, CAS 1394371-71-1 AMPK activator PF739 Aobious AOB33584, CAS 1852452-14-2 ER stress inducer Thapsigargin Sigma-Aldrich T9033, CAS 67526-95-8 AMPK inhibitor Compound C Selleck chemicals S7306, CAS 1219168-18-9 Bcl2 inhibitor Venetoclax Selleck chemicals S8048, CAS 1257044-40-8 6-Bn-ATP-g-S Biolog Life Science Institute B072, CAS 944834-42-8 p- Nitrobenzyl Mesylate Abcam ab138910, CAS 39628-94-9 GST-tagged human recombinant PERK Thermo-Fischer Scientifc PV5106 GST-tagged human recombinant ULK-1 Thermo-Fischer Scientifc PV6430 GST-tagged human recombinant RSK-2 Thermo-Fischer Scientifc PV3323 PUMA2A New England Peptide EQWAREIGAQARRMAADLNA BAD New England Peptide LWAAQRYGRELRRMSDEFEGSFKGL W-HRK New England Peptide WSSAAQLTAARLKALGDELHQ BIM New England Peptide MRPEIWIAQELRRIGDEFNA PUMA New England Peptide EQWAREIGAQLRRMADDLNA NOXAA New England Peptide AELPPEFAAQLRKIGDKVYC (Continued on next page) e2 Cell Reports 38, 110197, January 4, 2022

    Article Title: RNA sequestration in P-bodies sustains myeloid leukemia
    Article Snippet: Primary human bone marrow CD34 + cells (STEMCELL Technologies cat. no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies cat. no 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies cat. no 2690).

    FACS:

    Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
    Article Snippet: Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions.

    Article Title: Cancer antigen targets and uses thereof
    Article Snippet: Primary human bone marrow CD34+ cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Flow-cytometric analysis The following antibodies were used to define antigen expression by flow-cytometry: CD82-PE (Biolegend, 342103); CD120b (TNF-RII)-PE (Miltenyi, 130–107-740); EMR2-FITC, -APC (Miltenyi, 130–104-654, 130–104-656); ITGB5-PE (Biolegend, 345203); CCR1-PE, -APC (Miltenyi, 130–100-367, 130–100-358); CD96-APC (Miltenyi, 130–101-031); PTPRJ (CD148)-PE (life technologies, A15799); CD70-PE, -FITC (Biolegend, 355104, 355106); CD85d (ILT4)-PE -APC (Miltenyi, 130–100-567, 130–100-559); LTB4R1-AF700 (Novus Biologicals, FAB099N); CD85h (ILT1)-APC (Miltenyi, 130–100-920); TLR2-APC (Miltenyi, 130–099-020); CR1 (aka CD35)-APC (Miltenyi, 130–099-923); ITGAX (CD11c)-APC (Biolegend, 301613); EMB (abcam, 179801); EMC10 (abcam PA5–25112); LILRB3-PE (Miltenyi, 130–101-662); LILRB4-APC (R&D, FAB24251A); DAGLB (abcam, PA5–26331); P2RY13 (Novus Biologicals, NBP2–37382); LILRA6-APC (Miltenyi, 130–101-665); SLC30A1 (Alomone labs, AZT-011); SLC6A6 (LSBio, LS-C179237); SEMA4A (R&D, FAB4694A); CD123-PE (BD Biosciences, 555644); CLEC12A-PE (Miltenyi, 130–106-482); CD33-APC (Miltenyi, 130–098-864); CD38-BV421 (BD Biosciences, 562444); CD34-PE/Cy7 (Biolegend, 343515); CD45RA-BV640 (Biolegend, 304135); CD90-FITC (BD Biosciences, 555595).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Primary human bone marrow CD34 + cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: RNA sequestration in P-bodies sustains myeloid leukaemia.
    Article Snippet: Srikanth Kodali , Ludovica Proietti , Gemma Valcarcel , Anna V. López-Rubio , Patrizia Pessina , Thomas Eder , Junchao Shi , Annie Jen , Núria Lupión-Garcia , Anne C. Starner, Mason D. Bartels, Yingzhi Cui , Caroline M. Sands , Ainoa Planas-Riverola, Alba Martínez , Talia Velasco-Hernandez , Laureano Tomás-Daza , Bernhard Alber , Gabriele Manhart, Isabella Maria Mayer , Karoline Kollmann , Alessandro Fatica , Pablo Menendez, Evgenia Shishkova, Rachel E. Rau, Biola M. Javierre , Joshua Coon , Qi Chen , Eric L. Van Nostrand , Jose L. Sardina , Florian Grebien & Bruno Di Stefano

    Article Title: Erythrocyte CD55 mediates the internalization of Plasmodium falciparum parasites
    Article Snippet: Bone-marrow-derived primary human CD34 +HSPCs (Stem Cell Technologies) were cultured in erythroid differentiation medium (cPIMDM) composed of Iscove Basal Medium (IMDM) (Biochrom) supplemented with 4 mM L-Glutamine (Sigma), 330 μg/ml holo-transferrin (BBI Solutions), 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix), 10 −6 M hydrocortisone (Sigma), 100 ng/ml SCF (R and D Systems), 5 ng/ml IL-3 (R and D Systems), 3 IU/ml Epo (Amgen) and 5% plasma (Octapharma) at 37°C in 5% CO 2 in air, as previously described ( ; ).

    Article Title: AMPK-PERK axis represses oxidative metabolism and enhances apoptotic priming of mitochondria in acute myeloid leukemia.
    Article Snippet: 2015-08-11-DC Primary Human bone marrow CD34+ normal hematopoietic cells StemCell Technologies 70002.2 Chemicals, peptides, and recombinant proteins AMPK activator GSK621 Selleck chemicals S7898, CAS 1346607-05-3 AMPK activator MK8722 Aobious AOB33226, CAS 1394371-71-1 AMPK activator PF739 Aobious AOB33584, CAS 1852452-14-2 ER stress inducer Thapsigargin Sigma-Aldrich T9033, CAS 67526-95-8 AMPK inhibitor Compound C Selleck chemicals S7306, CAS 1219168-18-9 Bcl2 inhibitor Venetoclax Selleck chemicals S8048, CAS 1257044-40-8 6-Bn-ATP-g-S Biolog Life Science Institute B072, CAS 944834-42-8 p- Nitrobenzyl Mesylate Abcam ab138910, CAS 39628-94-9 GST-tagged human recombinant PERK Thermo-Fischer Scientifc PV5106 GST-tagged human recombinant ULK-1 Thermo-Fischer Scientifc PV6430 GST-tagged human recombinant RSK-2 Thermo-Fischer Scientifc PV3323 PUMA2A New England Peptide EQWAREIGAQARRMAADLNA BAD New England Peptide LWAAQRYGRELRRMSDEFEGSFKGL W-HRK New England Peptide WSSAAQLTAARLKALGDELHQ BIM New England Peptide MRPEIWIAQELRRIGDEFNA PUMA New England Peptide EQWAREIGAQLRRMADDLNA NOXAA New England Peptide AELPPEFAAQLRKIGDKVYC (Continued on next page) e2 Cell Reports 38, 110197, January 4, 2022

    Article Title: RNA sequestration in P-bodies sustains myeloid leukemia
    Article Snippet: Primary human bone marrow CD34 + cells (STEMCELL Technologies cat. no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies cat. no 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies cat. no 2690).

    Two Tailed Test:

    Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
    Article Snippet: Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions.

    Article Title: Cancer antigen targets and uses thereof
    Article Snippet: Primary human bone marrow CD34+ cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Flow-cytometric analysis The following antibodies were used to define antigen expression by flow-cytometry: CD82-PE (Biolegend, 342103); CD120b (TNF-RII)-PE (Miltenyi, 130–107-740); EMR2-FITC, -APC (Miltenyi, 130–104-654, 130–104-656); ITGB5-PE (Biolegend, 345203); CCR1-PE, -APC (Miltenyi, 130–100-367, 130–100-358); CD96-APC (Miltenyi, 130–101-031); PTPRJ (CD148)-PE (life technologies, A15799); CD70-PE, -FITC (Biolegend, 355104, 355106); CD85d (ILT4)-PE -APC (Miltenyi, 130–100-567, 130–100-559); LTB4R1-AF700 (Novus Biologicals, FAB099N); CD85h (ILT1)-APC (Miltenyi, 130–100-920); TLR2-APC (Miltenyi, 130–099-020); CR1 (aka CD35)-APC (Miltenyi, 130–099-923); ITGAX (CD11c)-APC (Biolegend, 301613); EMB (abcam, 179801); EMC10 (abcam PA5–25112); LILRB3-PE (Miltenyi, 130–101-662); LILRB4-APC (R&D, FAB24251A); DAGLB (abcam, PA5–26331); P2RY13 (Novus Biologicals, NBP2–37382); LILRA6-APC (Miltenyi, 130–101-665); SLC30A1 (Alomone labs, AZT-011); SLC6A6 (LSBio, LS-C179237); SEMA4A (R&D, FAB4694A); CD123-PE (BD Biosciences, 555644); CLEC12A-PE (Miltenyi, 130–106-482); CD33-APC (Miltenyi, 130–098-864); CD38-BV421 (BD Biosciences, 562444); CD34-PE/Cy7 (Biolegend, 343515); CD45RA-BV640 (Biolegend, 304135); CD90-FITC (BD Biosciences, 555595).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Primary human bone marrow CD34 + cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: RNA sequestration in P-bodies sustains myeloid leukaemia.
    Article Snippet: Srikanth Kodali , Ludovica Proietti , Gemma Valcarcel , Anna V. López-Rubio , Patrizia Pessina , Thomas Eder , Junchao Shi , Annie Jen , Núria Lupión-Garcia , Anne C. Starner, Mason D. Bartels, Yingzhi Cui , Caroline M. Sands , Ainoa Planas-Riverola, Alba Martínez , Talia Velasco-Hernandez , Laureano Tomás-Daza , Bernhard Alber , Gabriele Manhart, Isabella Maria Mayer , Karoline Kollmann , Alessandro Fatica , Pablo Menendez, Evgenia Shishkova, Rachel E. Rau, Biola M. Javierre , Joshua Coon , Qi Chen , Eric L. Van Nostrand , Jose L. Sardina , Florian Grebien & Bruno Di Stefano

    Article Title: Erythrocyte CD55 mediates the internalization of Plasmodium falciparum parasites
    Article Snippet: Bone-marrow-derived primary human CD34 +HSPCs (Stem Cell Technologies) were cultured in erythroid differentiation medium (cPIMDM) composed of Iscove Basal Medium (IMDM) (Biochrom) supplemented with 4 mM L-Glutamine (Sigma), 330 μg/ml holo-transferrin (BBI Solutions), 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix), 10 −6 M hydrocortisone (Sigma), 100 ng/ml SCF (R and D Systems), 5 ng/ml IL-3 (R and D Systems), 3 IU/ml Epo (Amgen) and 5% plasma (Octapharma) at 37°C in 5% CO 2 in air, as previously described ( ; ).

    Article Title: AMPK-PERK axis represses oxidative metabolism and enhances apoptotic priming of mitochondria in acute myeloid leukemia.
    Article Snippet: 2015-08-11-DC Primary Human bone marrow CD34+ normal hematopoietic cells StemCell Technologies 70002.2 Chemicals, peptides, and recombinant proteins AMPK activator GSK621 Selleck chemicals S7898, CAS 1346607-05-3 AMPK activator MK8722 Aobious AOB33226, CAS 1394371-71-1 AMPK activator PF739 Aobious AOB33584, CAS 1852452-14-2 ER stress inducer Thapsigargin Sigma-Aldrich T9033, CAS 67526-95-8 AMPK inhibitor Compound C Selleck chemicals S7306, CAS 1219168-18-9 Bcl2 inhibitor Venetoclax Selleck chemicals S8048, CAS 1257044-40-8 6-Bn-ATP-g-S Biolog Life Science Institute B072, CAS 944834-42-8 p- Nitrobenzyl Mesylate Abcam ab138910, CAS 39628-94-9 GST-tagged human recombinant PERK Thermo-Fischer Scientifc PV5106 GST-tagged human recombinant ULK-1 Thermo-Fischer Scientifc PV6430 GST-tagged human recombinant RSK-2 Thermo-Fischer Scientifc PV3323 PUMA2A New England Peptide EQWAREIGAQARRMAADLNA BAD New England Peptide LWAAQRYGRELRRMSDEFEGSFKGL W-HRK New England Peptide WSSAAQLTAARLKALGDELHQ BIM New England Peptide MRPEIWIAQELRRIGDEFNA PUMA New England Peptide EQWAREIGAQLRRMADDLNA NOXAA New England Peptide AELPPEFAAQLRKIGDKVYC (Continued on next page) e2 Cell Reports 38, 110197, January 4, 2022

    Article Title: RNA sequestration in P-bodies sustains myeloid leukemia
    Article Snippet: Primary human bone marrow CD34 + cells (STEMCELL Technologies cat. no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies cat. no 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies cat. no 2690).

    Derivative Assay:

    Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
    Article Snippet: Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions.

    Article Title: Cancer antigen targets and uses thereof
    Article Snippet: Primary human bone marrow CD34+ cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Flow-cytometric analysis The following antibodies were used to define antigen expression by flow-cytometry: CD82-PE (Biolegend, 342103); CD120b (TNF-RII)-PE (Miltenyi, 130–107-740); EMR2-FITC, -APC (Miltenyi, 130–104-654, 130–104-656); ITGB5-PE (Biolegend, 345203); CCR1-PE, -APC (Miltenyi, 130–100-367, 130–100-358); CD96-APC (Miltenyi, 130–101-031); PTPRJ (CD148)-PE (life technologies, A15799); CD70-PE, -FITC (Biolegend, 355104, 355106); CD85d (ILT4)-PE -APC (Miltenyi, 130–100-567, 130–100-559); LTB4R1-AF700 (Novus Biologicals, FAB099N); CD85h (ILT1)-APC (Miltenyi, 130–100-920); TLR2-APC (Miltenyi, 130–099-020); CR1 (aka CD35)-APC (Miltenyi, 130–099-923); ITGAX (CD11c)-APC (Biolegend, 301613); EMB (abcam, 179801); EMC10 (abcam PA5–25112); LILRB3-PE (Miltenyi, 130–101-662); LILRB4-APC (R&D, FAB24251A); DAGLB (abcam, PA5–26331); P2RY13 (Novus Biologicals, NBP2–37382); LILRA6-APC (Miltenyi, 130–101-665); SLC30A1 (Alomone labs, AZT-011); SLC6A6 (LSBio, LS-C179237); SEMA4A (R&D, FAB4694A); CD123-PE (BD Biosciences, 555644); CLEC12A-PE (Miltenyi, 130–106-482); CD33-APC (Miltenyi, 130–098-864); CD38-BV421 (BD Biosciences, 562444); CD34-PE/Cy7 (Biolegend, 343515); CD45RA-BV640 (Biolegend, 304135); CD90-FITC (BD Biosciences, 555595).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Primary human bone marrow CD34 + cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: RNA sequestration in P-bodies sustains myeloid leukaemia.
    Article Snippet: Srikanth Kodali , Ludovica Proietti , Gemma Valcarcel , Anna V. López-Rubio , Patrizia Pessina , Thomas Eder , Junchao Shi , Annie Jen , Núria Lupión-Garcia , Anne C. Starner, Mason D. Bartels, Yingzhi Cui , Caroline M. Sands , Ainoa Planas-Riverola, Alba Martínez , Talia Velasco-Hernandez , Laureano Tomás-Daza , Bernhard Alber , Gabriele Manhart, Isabella Maria Mayer , Karoline Kollmann , Alessandro Fatica , Pablo Menendez, Evgenia Shishkova, Rachel E. Rau, Biola M. Javierre , Joshua Coon , Qi Chen , Eric L. Van Nostrand , Jose L. Sardina , Florian Grebien & Bruno Di Stefano

    Article Title: Erythrocyte CD55 mediates the internalization of Plasmodium falciparum parasites
    Article Snippet: Bone-marrow-derived primary human CD34 +HSPCs (Stem Cell Technologies) were cultured in erythroid differentiation medium (cPIMDM) composed of Iscove Basal Medium (IMDM) (Biochrom) supplemented with 4 mM L-Glutamine (Sigma), 330 μg/ml holo-transferrin (BBI Solutions), 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix), 10 −6 M hydrocortisone (Sigma), 100 ng/ml SCF (R and D Systems), 5 ng/ml IL-3 (R and D Systems), 3 IU/ml Epo (Amgen) and 5% plasma (Octapharma) at 37°C in 5% CO 2 in air, as previously described ( ; ).

    Article Title: AMPK-PERK axis represses oxidative metabolism and enhances apoptotic priming of mitochondria in acute myeloid leukemia.
    Article Snippet: 2015-08-11-DC Primary Human bone marrow CD34+ normal hematopoietic cells StemCell Technologies 70002.2 Chemicals, peptides, and recombinant proteins AMPK activator GSK621 Selleck chemicals S7898, CAS 1346607-05-3 AMPK activator MK8722 Aobious AOB33226, CAS 1394371-71-1 AMPK activator PF739 Aobious AOB33584, CAS 1852452-14-2 ER stress inducer Thapsigargin Sigma-Aldrich T9033, CAS 67526-95-8 AMPK inhibitor Compound C Selleck chemicals S7306, CAS 1219168-18-9 Bcl2 inhibitor Venetoclax Selleck chemicals S8048, CAS 1257044-40-8 6-Bn-ATP-g-S Biolog Life Science Institute B072, CAS 944834-42-8 p- Nitrobenzyl Mesylate Abcam ab138910, CAS 39628-94-9 GST-tagged human recombinant PERK Thermo-Fischer Scientifc PV5106 GST-tagged human recombinant ULK-1 Thermo-Fischer Scientifc PV6430 GST-tagged human recombinant RSK-2 Thermo-Fischer Scientifc PV3323 PUMA2A New England Peptide EQWAREIGAQARRMAADLNA BAD New England Peptide LWAAQRYGRELRRMSDEFEGSFKGL W-HRK New England Peptide WSSAAQLTAARLKALGDELHQ BIM New England Peptide MRPEIWIAQELRRIGDEFNA PUMA New England Peptide EQWAREIGAQLRRMADDLNA NOXAA New England Peptide AELPPEFAAQLRKIGDKVYC (Continued on next page) e2 Cell Reports 38, 110197, January 4, 2022

    Article Title: RNA sequestration in P-bodies sustains myeloid leukemia
    Article Snippet: Primary human bone marrow CD34 + cells (STEMCELL Technologies cat. no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies cat. no 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies cat. no 2690).

    Comparison:

    Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
    Article Snippet: Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions.

    Article Title: Cancer antigen targets and uses thereof
    Article Snippet: Primary human bone marrow CD34+ cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Flow-cytometric analysis The following antibodies were used to define antigen expression by flow-cytometry: CD82-PE (Biolegend, 342103); CD120b (TNF-RII)-PE (Miltenyi, 130–107-740); EMR2-FITC, -APC (Miltenyi, 130–104-654, 130–104-656); ITGB5-PE (Biolegend, 345203); CCR1-PE, -APC (Miltenyi, 130–100-367, 130–100-358); CD96-APC (Miltenyi, 130–101-031); PTPRJ (CD148)-PE (life technologies, A15799); CD70-PE, -FITC (Biolegend, 355104, 355106); CD85d (ILT4)-PE -APC (Miltenyi, 130–100-567, 130–100-559); LTB4R1-AF700 (Novus Biologicals, FAB099N); CD85h (ILT1)-APC (Miltenyi, 130–100-920); TLR2-APC (Miltenyi, 130–099-020); CR1 (aka CD35)-APC (Miltenyi, 130–099-923); ITGAX (CD11c)-APC (Biolegend, 301613); EMB (abcam, 179801); EMC10 (abcam PA5–25112); LILRB3-PE (Miltenyi, 130–101-662); LILRB4-APC (R&D, FAB24251A); DAGLB (abcam, PA5–26331); P2RY13 (Novus Biologicals, NBP2–37382); LILRA6-APC (Miltenyi, 130–101-665); SLC30A1 (Alomone labs, AZT-011); SLC6A6 (LSBio, LS-C179237); SEMA4A (R&D, FAB4694A); CD123-PE (BD Biosciences, 555644); CLEC12A-PE (Miltenyi, 130–106-482); CD33-APC (Miltenyi, 130–098-864); CD38-BV421 (BD Biosciences, 562444); CD34-PE/Cy7 (Biolegend, 343515); CD45RA-BV640 (Biolegend, 304135); CD90-FITC (BD Biosciences, 555595).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Primary human bone marrow CD34 + cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: RNA sequestration in P-bodies sustains myeloid leukaemia.
    Article Snippet: Srikanth Kodali , Ludovica Proietti , Gemma Valcarcel , Anna V. López-Rubio , Patrizia Pessina , Thomas Eder , Junchao Shi , Annie Jen , Núria Lupión-Garcia , Anne C. Starner, Mason D. Bartels, Yingzhi Cui , Caroline M. Sands , Ainoa Planas-Riverola, Alba Martínez , Talia Velasco-Hernandez , Laureano Tomás-Daza , Bernhard Alber , Gabriele Manhart, Isabella Maria Mayer , Karoline Kollmann , Alessandro Fatica , Pablo Menendez, Evgenia Shishkova, Rachel E. Rau, Biola M. Javierre , Joshua Coon , Qi Chen , Eric L. Van Nostrand , Jose L. Sardina , Florian Grebien & Bruno Di Stefano

    Article Title: Erythrocyte CD55 mediates the internalization of Plasmodium falciparum parasites
    Article Snippet: Bone-marrow-derived primary human CD34 +HSPCs (Stem Cell Technologies) were cultured in erythroid differentiation medium (cPIMDM) composed of Iscove Basal Medium (IMDM) (Biochrom) supplemented with 4 mM L-Glutamine (Sigma), 330 μg/ml holo-transferrin (BBI Solutions), 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix), 10 −6 M hydrocortisone (Sigma), 100 ng/ml SCF (R and D Systems), 5 ng/ml IL-3 (R and D Systems), 3 IU/ml Epo (Amgen) and 5% plasma (Octapharma) at 37°C in 5% CO 2 in air, as previously described ( ; ).

    Article Title: AMPK-PERK axis represses oxidative metabolism and enhances apoptotic priming of mitochondria in acute myeloid leukemia.
    Article Snippet: 2015-08-11-DC Primary Human bone marrow CD34+ normal hematopoietic cells StemCell Technologies 70002.2 Chemicals, peptides, and recombinant proteins AMPK activator GSK621 Selleck chemicals S7898, CAS 1346607-05-3 AMPK activator MK8722 Aobious AOB33226, CAS 1394371-71-1 AMPK activator PF739 Aobious AOB33584, CAS 1852452-14-2 ER stress inducer Thapsigargin Sigma-Aldrich T9033, CAS 67526-95-8 AMPK inhibitor Compound C Selleck chemicals S7306, CAS 1219168-18-9 Bcl2 inhibitor Venetoclax Selleck chemicals S8048, CAS 1257044-40-8 6-Bn-ATP-g-S Biolog Life Science Institute B072, CAS 944834-42-8 p- Nitrobenzyl Mesylate Abcam ab138910, CAS 39628-94-9 GST-tagged human recombinant PERK Thermo-Fischer Scientifc PV5106 GST-tagged human recombinant ULK-1 Thermo-Fischer Scientifc PV6430 GST-tagged human recombinant RSK-2 Thermo-Fischer Scientifc PV3323 PUMA2A New England Peptide EQWAREIGAQARRMAADLNA BAD New England Peptide LWAAQRYGRELRRMSDEFEGSFKGL W-HRK New England Peptide WSSAAQLTAARLKALGDELHQ BIM New England Peptide MRPEIWIAQELRRIGDEFNA PUMA New England Peptide EQWAREIGAQLRRMADDLNA NOXAA New England Peptide AELPPEFAAQLRKIGDKVYC (Continued on next page) e2 Cell Reports 38, 110197, January 4, 2022

    Article Title: RNA sequestration in P-bodies sustains myeloid leukemia
    Article Snippet: Primary human bone marrow CD34 + cells (STEMCELL Technologies cat. no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies cat. no 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies cat. no 2690).

    Transduction:

    Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
    Article Snippet: Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions.

    Article Title: Cancer antigen targets and uses thereof
    Article Snippet: Primary human bone marrow CD34+ cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Flow-cytometric analysis The following antibodies were used to define antigen expression by flow-cytometry: CD82-PE (Biolegend, 342103); CD120b (TNF-RII)-PE (Miltenyi, 130–107-740); EMR2-FITC, -APC (Miltenyi, 130–104-654, 130–104-656); ITGB5-PE (Biolegend, 345203); CCR1-PE, -APC (Miltenyi, 130–100-367, 130–100-358); CD96-APC (Miltenyi, 130–101-031); PTPRJ (CD148)-PE (life technologies, A15799); CD70-PE, -FITC (Biolegend, 355104, 355106); CD85d (ILT4)-PE -APC (Miltenyi, 130–100-567, 130–100-559); LTB4R1-AF700 (Novus Biologicals, FAB099N); CD85h (ILT1)-APC (Miltenyi, 130–100-920); TLR2-APC (Miltenyi, 130–099-020); CR1 (aka CD35)-APC (Miltenyi, 130–099-923); ITGAX (CD11c)-APC (Biolegend, 301613); EMB (abcam, 179801); EMC10 (abcam PA5–25112); LILRB3-PE (Miltenyi, 130–101-662); LILRB4-APC (R&D, FAB24251A); DAGLB (abcam, PA5–26331); P2RY13 (Novus Biologicals, NBP2–37382); LILRA6-APC (Miltenyi, 130–101-665); SLC30A1 (Alomone labs, AZT-011); SLC6A6 (LSBio, LS-C179237); SEMA4A (R&D, FAB4694A); CD123-PE (BD Biosciences, 555644); CLEC12A-PE (Miltenyi, 130–106-482); CD33-APC (Miltenyi, 130–098-864); CD38-BV421 (BD Biosciences, 562444); CD34-PE/Cy7 (Biolegend, 343515); CD45RA-BV640 (Biolegend, 304135); CD90-FITC (BD Biosciences, 555595).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Primary human bone marrow CD34 + cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: RNA sequestration in P-bodies sustains myeloid leukaemia.
    Article Snippet: Srikanth Kodali , Ludovica Proietti , Gemma Valcarcel , Anna V. López-Rubio , Patrizia Pessina , Thomas Eder , Junchao Shi , Annie Jen , Núria Lupión-Garcia , Anne C. Starner, Mason D. Bartels, Yingzhi Cui , Caroline M. Sands , Ainoa Planas-Riverola, Alba Martínez , Talia Velasco-Hernandez , Laureano Tomás-Daza , Bernhard Alber , Gabriele Manhart, Isabella Maria Mayer , Karoline Kollmann , Alessandro Fatica , Pablo Menendez, Evgenia Shishkova, Rachel E. Rau, Biola M. Javierre , Joshua Coon , Qi Chen , Eric L. Van Nostrand , Jose L. Sardina , Florian Grebien & Bruno Di Stefano

    Article Title: Erythrocyte CD55 mediates the internalization of Plasmodium falciparum parasites
    Article Snippet: Bone-marrow-derived primary human CD34 +HSPCs (Stem Cell Technologies) were cultured in erythroid differentiation medium (cPIMDM) composed of Iscove Basal Medium (IMDM) (Biochrom) supplemented with 4 mM L-Glutamine (Sigma), 330 μg/ml holo-transferrin (BBI Solutions), 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix), 10 −6 M hydrocortisone (Sigma), 100 ng/ml SCF (R and D Systems), 5 ng/ml IL-3 (R and D Systems), 3 IU/ml Epo (Amgen) and 5% plasma (Octapharma) at 37°C in 5% CO 2 in air, as previously described ( ; ).

    Article Title: AMPK-PERK axis represses oxidative metabolism and enhances apoptotic priming of mitochondria in acute myeloid leukemia.
    Article Snippet: 2015-08-11-DC Primary Human bone marrow CD34+ normal hematopoietic cells StemCell Technologies 70002.2 Chemicals, peptides, and recombinant proteins AMPK activator GSK621 Selleck chemicals S7898, CAS 1346607-05-3 AMPK activator MK8722 Aobious AOB33226, CAS 1394371-71-1 AMPK activator PF739 Aobious AOB33584, CAS 1852452-14-2 ER stress inducer Thapsigargin Sigma-Aldrich T9033, CAS 67526-95-8 AMPK inhibitor Compound C Selleck chemicals S7306, CAS 1219168-18-9 Bcl2 inhibitor Venetoclax Selleck chemicals S8048, CAS 1257044-40-8 6-Bn-ATP-g-S Biolog Life Science Institute B072, CAS 944834-42-8 p- Nitrobenzyl Mesylate Abcam ab138910, CAS 39628-94-9 GST-tagged human recombinant PERK Thermo-Fischer Scientifc PV5106 GST-tagged human recombinant ULK-1 Thermo-Fischer Scientifc PV6430 GST-tagged human recombinant RSK-2 Thermo-Fischer Scientifc PV3323 PUMA2A New England Peptide EQWAREIGAQARRMAADLNA BAD New England Peptide LWAAQRYGRELRRMSDEFEGSFKGL W-HRK New England Peptide WSSAAQLTAARLKALGDELHQ BIM New England Peptide MRPEIWIAQELRRIGDEFNA PUMA New England Peptide EQWAREIGAQLRRMADDLNA NOXAA New England Peptide AELPPEFAAQLRKIGDKVYC (Continued on next page) e2 Cell Reports 38, 110197, January 4, 2022

    Article Title: RNA sequestration in P-bodies sustains myeloid leukemia
    Article Snippet: Primary human bone marrow CD34 + cells (STEMCELL Technologies cat. no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies cat. no 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies cat. no 2690).

    Colony-forming Unit Assay:

    Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
    Article Snippet: Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions.

    Article Title: Cancer antigen targets and uses thereof
    Article Snippet: Primary human bone marrow CD34+ cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Flow-cytometric analysis The following antibodies were used to define antigen expression by flow-cytometry: CD82-PE (Biolegend, 342103); CD120b (TNF-RII)-PE (Miltenyi, 130–107-740); EMR2-FITC, -APC (Miltenyi, 130–104-654, 130–104-656); ITGB5-PE (Biolegend, 345203); CCR1-PE, -APC (Miltenyi, 130–100-367, 130–100-358); CD96-APC (Miltenyi, 130–101-031); PTPRJ (CD148)-PE (life technologies, A15799); CD70-PE, -FITC (Biolegend, 355104, 355106); CD85d (ILT4)-PE -APC (Miltenyi, 130–100-567, 130–100-559); LTB4R1-AF700 (Novus Biologicals, FAB099N); CD85h (ILT1)-APC (Miltenyi, 130–100-920); TLR2-APC (Miltenyi, 130–099-020); CR1 (aka CD35)-APC (Miltenyi, 130–099-923); ITGAX (CD11c)-APC (Biolegend, 301613); EMB (abcam, 179801); EMC10 (abcam PA5–25112); LILRB3-PE (Miltenyi, 130–101-662); LILRB4-APC (R&D, FAB24251A); DAGLB (abcam, PA5–26331); P2RY13 (Novus Biologicals, NBP2–37382); LILRA6-APC (Miltenyi, 130–101-665); SLC30A1 (Alomone labs, AZT-011); SLC6A6 (LSBio, LS-C179237); SEMA4A (R&D, FAB4694A); CD123-PE (BD Biosciences, 555644); CLEC12A-PE (Miltenyi, 130–106-482); CD33-APC (Miltenyi, 130–098-864); CD38-BV421 (BD Biosciences, 562444); CD34-PE/Cy7 (Biolegend, 343515); CD45RA-BV640 (Biolegend, 304135); CD90-FITC (BD Biosciences, 555595).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Primary human bone marrow CD34 + cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: RNA sequestration in P-bodies sustains myeloid leukaemia.
    Article Snippet: Srikanth Kodali , Ludovica Proietti , Gemma Valcarcel , Anna V. López-Rubio , Patrizia Pessina , Thomas Eder , Junchao Shi , Annie Jen , Núria Lupión-Garcia , Anne C. Starner, Mason D. Bartels, Yingzhi Cui , Caroline M. Sands , Ainoa Planas-Riverola, Alba Martínez , Talia Velasco-Hernandez , Laureano Tomás-Daza , Bernhard Alber , Gabriele Manhart, Isabella Maria Mayer , Karoline Kollmann , Alessandro Fatica , Pablo Menendez, Evgenia Shishkova, Rachel E. Rau, Biola M. Javierre , Joshua Coon , Qi Chen , Eric L. Van Nostrand , Jose L. Sardina , Florian Grebien & Bruno Di Stefano

    Article Title: Erythrocyte CD55 mediates the internalization of Plasmodium falciparum parasites
    Article Snippet: Bone-marrow-derived primary human CD34 +HSPCs (Stem Cell Technologies) were cultured in erythroid differentiation medium (cPIMDM) composed of Iscove Basal Medium (IMDM) (Biochrom) supplemented with 4 mM L-Glutamine (Sigma), 330 μg/ml holo-transferrin (BBI Solutions), 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix), 10 −6 M hydrocortisone (Sigma), 100 ng/ml SCF (R and D Systems), 5 ng/ml IL-3 (R and D Systems), 3 IU/ml Epo (Amgen) and 5% plasma (Octapharma) at 37°C in 5% CO 2 in air, as previously described ( ; ).

    Article Title: AMPK-PERK axis represses oxidative metabolism and enhances apoptotic priming of mitochondria in acute myeloid leukemia.
    Article Snippet: 2015-08-11-DC Primary Human bone marrow CD34+ normal hematopoietic cells StemCell Technologies 70002.2 Chemicals, peptides, and recombinant proteins AMPK activator GSK621 Selleck chemicals S7898, CAS 1346607-05-3 AMPK activator MK8722 Aobious AOB33226, CAS 1394371-71-1 AMPK activator PF739 Aobious AOB33584, CAS 1852452-14-2 ER stress inducer Thapsigargin Sigma-Aldrich T9033, CAS 67526-95-8 AMPK inhibitor Compound C Selleck chemicals S7306, CAS 1219168-18-9 Bcl2 inhibitor Venetoclax Selleck chemicals S8048, CAS 1257044-40-8 6-Bn-ATP-g-S Biolog Life Science Institute B072, CAS 944834-42-8 p- Nitrobenzyl Mesylate Abcam ab138910, CAS 39628-94-9 GST-tagged human recombinant PERK Thermo-Fischer Scientifc PV5106 GST-tagged human recombinant ULK-1 Thermo-Fischer Scientifc PV6430 GST-tagged human recombinant RSK-2 Thermo-Fischer Scientifc PV3323 PUMA2A New England Peptide EQWAREIGAQARRMAADLNA BAD New England Peptide LWAAQRYGRELRRMSDEFEGSFKGL W-HRK New England Peptide WSSAAQLTAARLKALGDELHQ BIM New England Peptide MRPEIWIAQELRRIGDEFNA PUMA New England Peptide EQWAREIGAQLRRMADDLNA NOXAA New England Peptide AELPPEFAAQLRKIGDKVYC (Continued on next page) e2 Cell Reports 38, 110197, January 4, 2022

    Article Title: RNA sequestration in P-bodies sustains myeloid leukemia
    Article Snippet: Primary human bone marrow CD34 + cells (STEMCELL Technologies cat. no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies cat. no 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies cat. no 2690).

    Recombinant:

    Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
    Article Snippet: Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions.

    Article Title: Cancer antigen targets and uses thereof
    Article Snippet: Primary human bone marrow CD34+ cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Flow-cytometric analysis The following antibodies were used to define antigen expression by flow-cytometry: CD82-PE (Biolegend, 342103); CD120b (TNF-RII)-PE (Miltenyi, 130–107-740); EMR2-FITC, -APC (Miltenyi, 130–104-654, 130–104-656); ITGB5-PE (Biolegend, 345203); CCR1-PE, -APC (Miltenyi, 130–100-367, 130–100-358); CD96-APC (Miltenyi, 130–101-031); PTPRJ (CD148)-PE (life technologies, A15799); CD70-PE, -FITC (Biolegend, 355104, 355106); CD85d (ILT4)-PE -APC (Miltenyi, 130–100-567, 130–100-559); LTB4R1-AF700 (Novus Biologicals, FAB099N); CD85h (ILT1)-APC (Miltenyi, 130–100-920); TLR2-APC (Miltenyi, 130–099-020); CR1 (aka CD35)-APC (Miltenyi, 130–099-923); ITGAX (CD11c)-APC (Biolegend, 301613); EMB (abcam, 179801); EMC10 (abcam PA5–25112); LILRB3-PE (Miltenyi, 130–101-662); LILRB4-APC (R&D, FAB24251A); DAGLB (abcam, PA5–26331); P2RY13 (Novus Biologicals, NBP2–37382); LILRA6-APC (Miltenyi, 130–101-665); SLC30A1 (Alomone labs, AZT-011); SLC6A6 (LSBio, LS-C179237); SEMA4A (R&D, FAB4694A); CD123-PE (BD Biosciences, 555644); CLEC12A-PE (Miltenyi, 130–106-482); CD33-APC (Miltenyi, 130–098-864); CD38-BV421 (BD Biosciences, 562444); CD34-PE/Cy7 (Biolegend, 343515); CD45RA-BV640 (Biolegend, 304135); CD90-FITC (BD Biosciences, 555595).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Primary human bone marrow CD34 + cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: RNA sequestration in P-bodies sustains myeloid leukaemia.
    Article Snippet: Srikanth Kodali , Ludovica Proietti , Gemma Valcarcel , Anna V. López-Rubio , Patrizia Pessina , Thomas Eder , Junchao Shi , Annie Jen , Núria Lupión-Garcia , Anne C. Starner, Mason D. Bartels, Yingzhi Cui , Caroline M. Sands , Ainoa Planas-Riverola, Alba Martínez , Talia Velasco-Hernandez , Laureano Tomás-Daza , Bernhard Alber , Gabriele Manhart, Isabella Maria Mayer , Karoline Kollmann , Alessandro Fatica , Pablo Menendez, Evgenia Shishkova, Rachel E. Rau, Biola M. Javierre , Joshua Coon , Qi Chen , Eric L. Van Nostrand , Jose L. Sardina , Florian Grebien & Bruno Di Stefano

    Article Title: Erythrocyte CD55 mediates the internalization of Plasmodium falciparum parasites
    Article Snippet: Bone-marrow-derived primary human CD34 +HSPCs (Stem Cell Technologies) were cultured in erythroid differentiation medium (cPIMDM) composed of Iscove Basal Medium (IMDM) (Biochrom) supplemented with 4 mM L-Glutamine (Sigma), 330 μg/ml holo-transferrin (BBI Solutions), 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix), 10 −6 M hydrocortisone (Sigma), 100 ng/ml SCF (R and D Systems), 5 ng/ml IL-3 (R and D Systems), 3 IU/ml Epo (Amgen) and 5% plasma (Octapharma) at 37°C in 5% CO 2 in air, as previously described ( ; ).

    Article Title: AMPK-PERK axis represses oxidative metabolism and enhances apoptotic priming of mitochondria in acute myeloid leukemia.
    Article Snippet: 2015-08-11-DC Primary Human bone marrow CD34+ normal hematopoietic cells StemCell Technologies 70002.2 Chemicals, peptides, and recombinant proteins AMPK activator GSK621 Selleck chemicals S7898, CAS 1346607-05-3 AMPK activator MK8722 Aobious AOB33226, CAS 1394371-71-1 AMPK activator PF739 Aobious AOB33584, CAS 1852452-14-2 ER stress inducer Thapsigargin Sigma-Aldrich T9033, CAS 67526-95-8 AMPK inhibitor Compound C Selleck chemicals S7306, CAS 1219168-18-9 Bcl2 inhibitor Venetoclax Selleck chemicals S8048, CAS 1257044-40-8 6-Bn-ATP-g-S Biolog Life Science Institute B072, CAS 944834-42-8 p- Nitrobenzyl Mesylate Abcam ab138910, CAS 39628-94-9 GST-tagged human recombinant PERK Thermo-Fischer Scientifc PV5106 GST-tagged human recombinant ULK-1 Thermo-Fischer Scientifc PV6430 GST-tagged human recombinant RSK-2 Thermo-Fischer Scientifc PV3323 PUMA2A New England Peptide EQWAREIGAQARRMAADLNA BAD New England Peptide LWAAQRYGRELRRMSDEFEGSFKGL W-HRK New England Peptide WSSAAQLTAARLKALGDELHQ BIM New England Peptide MRPEIWIAQELRRIGDEFNA PUMA New England Peptide EQWAREIGAQLRRMADDLNA NOXAA New England Peptide AELPPEFAAQLRKIGDKVYC (Continued on next page) e2 Cell Reports 38, 110197, January 4, 2022

    Article Title: RNA sequestration in P-bodies sustains myeloid leukemia
    Article Snippet: Primary human bone marrow CD34 + cells (STEMCELL Technologies cat. no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies cat. no 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies cat. no 2690).

    Blocking Assay:

    Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
    Article Snippet: Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions.

    Article Title: Cancer antigen targets and uses thereof
    Article Snippet: Primary human bone marrow CD34+ cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Flow-cytometric analysis The following antibodies were used to define antigen expression by flow-cytometry: CD82-PE (Biolegend, 342103); CD120b (TNF-RII)-PE (Miltenyi, 130–107-740); EMR2-FITC, -APC (Miltenyi, 130–104-654, 130–104-656); ITGB5-PE (Biolegend, 345203); CCR1-PE, -APC (Miltenyi, 130–100-367, 130–100-358); CD96-APC (Miltenyi, 130–101-031); PTPRJ (CD148)-PE (life technologies, A15799); CD70-PE, -FITC (Biolegend, 355104, 355106); CD85d (ILT4)-PE -APC (Miltenyi, 130–100-567, 130–100-559); LTB4R1-AF700 (Novus Biologicals, FAB099N); CD85h (ILT1)-APC (Miltenyi, 130–100-920); TLR2-APC (Miltenyi, 130–099-020); CR1 (aka CD35)-APC (Miltenyi, 130–099-923); ITGAX (CD11c)-APC (Biolegend, 301613); EMB (abcam, 179801); EMC10 (abcam PA5–25112); LILRB3-PE (Miltenyi, 130–101-662); LILRB4-APC (R&D, FAB24251A); DAGLB (abcam, PA5–26331); P2RY13 (Novus Biologicals, NBP2–37382); LILRA6-APC (Miltenyi, 130–101-665); SLC30A1 (Alomone labs, AZT-011); SLC6A6 (LSBio, LS-C179237); SEMA4A (R&D, FAB4694A); CD123-PE (BD Biosciences, 555644); CLEC12A-PE (Miltenyi, 130–106-482); CD33-APC (Miltenyi, 130–098-864); CD38-BV421 (BD Biosciences, 562444); CD34-PE/Cy7 (Biolegend, 343515); CD45RA-BV640 (Biolegend, 304135); CD90-FITC (BD Biosciences, 555595).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Primary human bone marrow CD34 + cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: RNA sequestration in P-bodies sustains myeloid leukaemia.
    Article Snippet: Srikanth Kodali , Ludovica Proietti , Gemma Valcarcel , Anna V. López-Rubio , Patrizia Pessina , Thomas Eder , Junchao Shi , Annie Jen , Núria Lupión-Garcia , Anne C. Starner, Mason D. Bartels, Yingzhi Cui , Caroline M. Sands , Ainoa Planas-Riverola, Alba Martínez , Talia Velasco-Hernandez , Laureano Tomás-Daza , Bernhard Alber , Gabriele Manhart, Isabella Maria Mayer , Karoline Kollmann , Alessandro Fatica , Pablo Menendez, Evgenia Shishkova, Rachel E. Rau, Biola M. Javierre , Joshua Coon , Qi Chen , Eric L. Van Nostrand , Jose L. Sardina , Florian Grebien & Bruno Di Stefano

    Article Title: Erythrocyte CD55 mediates the internalization of Plasmodium falciparum parasites
    Article Snippet: Bone-marrow-derived primary human CD34 +HSPCs (Stem Cell Technologies) were cultured in erythroid differentiation medium (cPIMDM) composed of Iscove Basal Medium (IMDM) (Biochrom) supplemented with 4 mM L-Glutamine (Sigma), 330 μg/ml holo-transferrin (BBI Solutions), 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix), 10 −6 M hydrocortisone (Sigma), 100 ng/ml SCF (R and D Systems), 5 ng/ml IL-3 (R and D Systems), 3 IU/ml Epo (Amgen) and 5% plasma (Octapharma) at 37°C in 5% CO 2 in air, as previously described ( ; ).

    Article Title: AMPK-PERK axis represses oxidative metabolism and enhances apoptotic priming of mitochondria in acute myeloid leukemia.
    Article Snippet: 2015-08-11-DC Primary Human bone marrow CD34+ normal hematopoietic cells StemCell Technologies 70002.2 Chemicals, peptides, and recombinant proteins AMPK activator GSK621 Selleck chemicals S7898, CAS 1346607-05-3 AMPK activator MK8722 Aobious AOB33226, CAS 1394371-71-1 AMPK activator PF739 Aobious AOB33584, CAS 1852452-14-2 ER stress inducer Thapsigargin Sigma-Aldrich T9033, CAS 67526-95-8 AMPK inhibitor Compound C Selleck chemicals S7306, CAS 1219168-18-9 Bcl2 inhibitor Venetoclax Selleck chemicals S8048, CAS 1257044-40-8 6-Bn-ATP-g-S Biolog Life Science Institute B072, CAS 944834-42-8 p- Nitrobenzyl Mesylate Abcam ab138910, CAS 39628-94-9 GST-tagged human recombinant PERK Thermo-Fischer Scientifc PV5106 GST-tagged human recombinant ULK-1 Thermo-Fischer Scientifc PV6430 GST-tagged human recombinant RSK-2 Thermo-Fischer Scientifc PV3323 PUMA2A New England Peptide EQWAREIGAQARRMAADLNA BAD New England Peptide LWAAQRYGRELRRMSDEFEGSFKGL W-HRK New England Peptide WSSAAQLTAARLKALGDELHQ BIM New England Peptide MRPEIWIAQELRRIGDEFNA PUMA New England Peptide EQWAREIGAQLRRMADDLNA NOXAA New England Peptide AELPPEFAAQLRKIGDKVYC (Continued on next page) e2 Cell Reports 38, 110197, January 4, 2022

    Article Title: RNA sequestration in P-bodies sustains myeloid leukemia
    Article Snippet: Primary human bone marrow CD34 + cells (STEMCELL Technologies cat. no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies cat. no 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies cat. no 2690).

    Generated:

    Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
    Article Snippet: Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions.

    Article Title: Cancer antigen targets and uses thereof
    Article Snippet: Primary human bone marrow CD34+ cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Flow-cytometric analysis The following antibodies were used to define antigen expression by flow-cytometry: CD82-PE (Biolegend, 342103); CD120b (TNF-RII)-PE (Miltenyi, 130–107-740); EMR2-FITC, -APC (Miltenyi, 130–104-654, 130–104-656); ITGB5-PE (Biolegend, 345203); CCR1-PE, -APC (Miltenyi, 130–100-367, 130–100-358); CD96-APC (Miltenyi, 130–101-031); PTPRJ (CD148)-PE (life technologies, A15799); CD70-PE, -FITC (Biolegend, 355104, 355106); CD85d (ILT4)-PE -APC (Miltenyi, 130–100-567, 130–100-559); LTB4R1-AF700 (Novus Biologicals, FAB099N); CD85h (ILT1)-APC (Miltenyi, 130–100-920); TLR2-APC (Miltenyi, 130–099-020); CR1 (aka CD35)-APC (Miltenyi, 130–099-923); ITGAX (CD11c)-APC (Biolegend, 301613); EMB (abcam, 179801); EMC10 (abcam PA5–25112); LILRB3-PE (Miltenyi, 130–101-662); LILRB4-APC (R&D, FAB24251A); DAGLB (abcam, PA5–26331); P2RY13 (Novus Biologicals, NBP2–37382); LILRA6-APC (Miltenyi, 130–101-665); SLC30A1 (Alomone labs, AZT-011); SLC6A6 (LSBio, LS-C179237); SEMA4A (R&D, FAB4694A); CD123-PE (BD Biosciences, 555644); CLEC12A-PE (Miltenyi, 130–106-482); CD33-APC (Miltenyi, 130–098-864); CD38-BV421 (BD Biosciences, 562444); CD34-PE/Cy7 (Biolegend, 343515); CD45RA-BV640 (Biolegend, 304135); CD90-FITC (BD Biosciences, 555595).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Primary human bone marrow CD34 + cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: RNA sequestration in P-bodies sustains myeloid leukaemia.
    Article Snippet: Srikanth Kodali , Ludovica Proietti , Gemma Valcarcel , Anna V. López-Rubio , Patrizia Pessina , Thomas Eder , Junchao Shi , Annie Jen , Núria Lupión-Garcia , Anne C. Starner, Mason D. Bartels, Yingzhi Cui , Caroline M. Sands , Ainoa Planas-Riverola, Alba Martínez , Talia Velasco-Hernandez , Laureano Tomás-Daza , Bernhard Alber , Gabriele Manhart, Isabella Maria Mayer , Karoline Kollmann , Alessandro Fatica , Pablo Menendez, Evgenia Shishkova, Rachel E. Rau, Biola M. Javierre , Joshua Coon , Qi Chen , Eric L. Van Nostrand , Jose L. Sardina , Florian Grebien & Bruno Di Stefano

    Article Title: Erythrocyte CD55 mediates the internalization of Plasmodium falciparum parasites
    Article Snippet: Bone-marrow-derived primary human CD34 +HSPCs (Stem Cell Technologies) were cultured in erythroid differentiation medium (cPIMDM) composed of Iscove Basal Medium (IMDM) (Biochrom) supplemented with 4 mM L-Glutamine (Sigma), 330 μg/ml holo-transferrin (BBI Solutions), 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix), 10 −6 M hydrocortisone (Sigma), 100 ng/ml SCF (R and D Systems), 5 ng/ml IL-3 (R and D Systems), 3 IU/ml Epo (Amgen) and 5% plasma (Octapharma) at 37°C in 5% CO 2 in air, as previously described ( ; ).

    Article Title: AMPK-PERK axis represses oxidative metabolism and enhances apoptotic priming of mitochondria in acute myeloid leukemia.
    Article Snippet: 2015-08-11-DC Primary Human bone marrow CD34+ normal hematopoietic cells StemCell Technologies 70002.2 Chemicals, peptides, and recombinant proteins AMPK activator GSK621 Selleck chemicals S7898, CAS 1346607-05-3 AMPK activator MK8722 Aobious AOB33226, CAS 1394371-71-1 AMPK activator PF739 Aobious AOB33584, CAS 1852452-14-2 ER stress inducer Thapsigargin Sigma-Aldrich T9033, CAS 67526-95-8 AMPK inhibitor Compound C Selleck chemicals S7306, CAS 1219168-18-9 Bcl2 inhibitor Venetoclax Selleck chemicals S8048, CAS 1257044-40-8 6-Bn-ATP-g-S Biolog Life Science Institute B072, CAS 944834-42-8 p- Nitrobenzyl Mesylate Abcam ab138910, CAS 39628-94-9 GST-tagged human recombinant PERK Thermo-Fischer Scientifc PV5106 GST-tagged human recombinant ULK-1 Thermo-Fischer Scientifc PV6430 GST-tagged human recombinant RSK-2 Thermo-Fischer Scientifc PV3323 PUMA2A New England Peptide EQWAREIGAQARRMAADLNA BAD New England Peptide LWAAQRYGRELRRMSDEFEGSFKGL W-HRK New England Peptide WSSAAQLTAARLKALGDELHQ BIM New England Peptide MRPEIWIAQELRRIGDEFNA PUMA New England Peptide EQWAREIGAQLRRMADDLNA NOXAA New England Peptide AELPPEFAAQLRKIGDKVYC (Continued on next page) e2 Cell Reports 38, 110197, January 4, 2022

    Article Title: RNA sequestration in P-bodies sustains myeloid leukemia
    Article Snippet: Primary human bone marrow CD34 + cells (STEMCELL Technologies cat. no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies cat. no 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies cat. no 2690).

    Affinity Purification:

    Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
    Article Snippet: Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions.

    Article Title: Cancer antigen targets and uses thereof
    Article Snippet: Primary human bone marrow CD34+ cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Flow-cytometric analysis The following antibodies were used to define antigen expression by flow-cytometry: CD82-PE (Biolegend, 342103); CD120b (TNF-RII)-PE (Miltenyi, 130–107-740); EMR2-FITC, -APC (Miltenyi, 130–104-654, 130–104-656); ITGB5-PE (Biolegend, 345203); CCR1-PE, -APC (Miltenyi, 130–100-367, 130–100-358); CD96-APC (Miltenyi, 130–101-031); PTPRJ (CD148)-PE (life technologies, A15799); CD70-PE, -FITC (Biolegend, 355104, 355106); CD85d (ILT4)-PE -APC (Miltenyi, 130–100-567, 130–100-559); LTB4R1-AF700 (Novus Biologicals, FAB099N); CD85h (ILT1)-APC (Miltenyi, 130–100-920); TLR2-APC (Miltenyi, 130–099-020); CR1 (aka CD35)-APC (Miltenyi, 130–099-923); ITGAX (CD11c)-APC (Biolegend, 301613); EMB (abcam, 179801); EMC10 (abcam PA5–25112); LILRB3-PE (Miltenyi, 130–101-662); LILRB4-APC (R&D, FAB24251A); DAGLB (abcam, PA5–26331); P2RY13 (Novus Biologicals, NBP2–37382); LILRA6-APC (Miltenyi, 130–101-665); SLC30A1 (Alomone labs, AZT-011); SLC6A6 (LSBio, LS-C179237); SEMA4A (R&D, FAB4694A); CD123-PE (BD Biosciences, 555644); CLEC12A-PE (Miltenyi, 130–106-482); CD33-APC (Miltenyi, 130–098-864); CD38-BV421 (BD Biosciences, 562444); CD34-PE/Cy7 (Biolegend, 343515); CD45RA-BV640 (Biolegend, 304135); CD90-FITC (BD Biosciences, 555595).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Primary human bone marrow CD34 + cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: RNA sequestration in P-bodies sustains myeloid leukaemia.
    Article Snippet: Srikanth Kodali , Ludovica Proietti , Gemma Valcarcel , Anna V. López-Rubio , Patrizia Pessina , Thomas Eder , Junchao Shi , Annie Jen , Núria Lupión-Garcia , Anne C. Starner, Mason D. Bartels, Yingzhi Cui , Caroline M. Sands , Ainoa Planas-Riverola, Alba Martínez , Talia Velasco-Hernandez , Laureano Tomás-Daza , Bernhard Alber , Gabriele Manhart, Isabella Maria Mayer , Karoline Kollmann , Alessandro Fatica , Pablo Menendez, Evgenia Shishkova, Rachel E. Rau, Biola M. Javierre , Joshua Coon , Qi Chen , Eric L. Van Nostrand , Jose L. Sardina , Florian Grebien & Bruno Di Stefano

    Article Title: Erythrocyte CD55 mediates the internalization of Plasmodium falciparum parasites
    Article Snippet: Bone-marrow-derived primary human CD34 +HSPCs (Stem Cell Technologies) were cultured in erythroid differentiation medium (cPIMDM) composed of Iscove Basal Medium (IMDM) (Biochrom) supplemented with 4 mM L-Glutamine (Sigma), 330 μg/ml holo-transferrin (BBI Solutions), 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix), 10 −6 M hydrocortisone (Sigma), 100 ng/ml SCF (R and D Systems), 5 ng/ml IL-3 (R and D Systems), 3 IU/ml Epo (Amgen) and 5% plasma (Octapharma) at 37°C in 5% CO 2 in air, as previously described ( ; ).

    Article Title: AMPK-PERK axis represses oxidative metabolism and enhances apoptotic priming of mitochondria in acute myeloid leukemia.
    Article Snippet: 2015-08-11-DC Primary Human bone marrow CD34+ normal hematopoietic cells StemCell Technologies 70002.2 Chemicals, peptides, and recombinant proteins AMPK activator GSK621 Selleck chemicals S7898, CAS 1346607-05-3 AMPK activator MK8722 Aobious AOB33226, CAS 1394371-71-1 AMPK activator PF739 Aobious AOB33584, CAS 1852452-14-2 ER stress inducer Thapsigargin Sigma-Aldrich T9033, CAS 67526-95-8 AMPK inhibitor Compound C Selleck chemicals S7306, CAS 1219168-18-9 Bcl2 inhibitor Venetoclax Selleck chemicals S8048, CAS 1257044-40-8 6-Bn-ATP-g-S Biolog Life Science Institute B072, CAS 944834-42-8 p- Nitrobenzyl Mesylate Abcam ab138910, CAS 39628-94-9 GST-tagged human recombinant PERK Thermo-Fischer Scientifc PV5106 GST-tagged human recombinant ULK-1 Thermo-Fischer Scientifc PV6430 GST-tagged human recombinant RSK-2 Thermo-Fischer Scientifc PV3323 PUMA2A New England Peptide EQWAREIGAQARRMAADLNA BAD New England Peptide LWAAQRYGRELRRMSDEFEGSFKGL W-HRK New England Peptide WSSAAQLTAARLKALGDELHQ BIM New England Peptide MRPEIWIAQELRRIGDEFNA PUMA New England Peptide EQWAREIGAQLRRMADDLNA NOXAA New England Peptide AELPPEFAAQLRKIGDKVYC (Continued on next page) e2 Cell Reports 38, 110197, January 4, 2022

    Article Title: RNA sequestration in P-bodies sustains myeloid leukemia
    Article Snippet: Primary human bone marrow CD34 + cells (STEMCELL Technologies cat. no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies cat. no 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies cat. no 2690).

    Growth Assay:

    Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
    Article Snippet: Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions.

    Article Title: Cancer antigen targets and uses thereof
    Article Snippet: Primary human bone marrow CD34+ cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Flow-cytometric analysis The following antibodies were used to define antigen expression by flow-cytometry: CD82-PE (Biolegend, 342103); CD120b (TNF-RII)-PE (Miltenyi, 130–107-740); EMR2-FITC, -APC (Miltenyi, 130–104-654, 130–104-656); ITGB5-PE (Biolegend, 345203); CCR1-PE, -APC (Miltenyi, 130–100-367, 130–100-358); CD96-APC (Miltenyi, 130–101-031); PTPRJ (CD148)-PE (life technologies, A15799); CD70-PE, -FITC (Biolegend, 355104, 355106); CD85d (ILT4)-PE -APC (Miltenyi, 130–100-567, 130–100-559); LTB4R1-AF700 (Novus Biologicals, FAB099N); CD85h (ILT1)-APC (Miltenyi, 130–100-920); TLR2-APC (Miltenyi, 130–099-020); CR1 (aka CD35)-APC (Miltenyi, 130–099-923); ITGAX (CD11c)-APC (Biolegend, 301613); EMB (abcam, 179801); EMC10 (abcam PA5–25112); LILRB3-PE (Miltenyi, 130–101-662); LILRB4-APC (R&D, FAB24251A); DAGLB (abcam, PA5–26331); P2RY13 (Novus Biologicals, NBP2–37382); LILRA6-APC (Miltenyi, 130–101-665); SLC30A1 (Alomone labs, AZT-011); SLC6A6 (LSBio, LS-C179237); SEMA4A (R&D, FAB4694A); CD123-PE (BD Biosciences, 555644); CLEC12A-PE (Miltenyi, 130–106-482); CD33-APC (Miltenyi, 130–098-864); CD38-BV421 (BD Biosciences, 562444); CD34-PE/Cy7 (Biolegend, 343515); CD45RA-BV640 (Biolegend, 304135); CD90-FITC (BD Biosciences, 555595).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Primary human bone marrow CD34 + cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: RNA sequestration in P-bodies sustains myeloid leukaemia.
    Article Snippet: Srikanth Kodali , Ludovica Proietti , Gemma Valcarcel , Anna V. López-Rubio , Patrizia Pessina , Thomas Eder , Junchao Shi , Annie Jen , Núria Lupión-Garcia , Anne C. Starner, Mason D. Bartels, Yingzhi Cui , Caroline M. Sands , Ainoa Planas-Riverola, Alba Martínez , Talia Velasco-Hernandez , Laureano Tomás-Daza , Bernhard Alber , Gabriele Manhart, Isabella Maria Mayer , Karoline Kollmann , Alessandro Fatica , Pablo Menendez, Evgenia Shishkova, Rachel E. Rau, Biola M. Javierre , Joshua Coon , Qi Chen , Eric L. Van Nostrand , Jose L. Sardina , Florian Grebien & Bruno Di Stefano

    Article Title: Erythrocyte CD55 mediates the internalization of Plasmodium falciparum parasites
    Article Snippet: Bone-marrow-derived primary human CD34 +HSPCs (Stem Cell Technologies) were cultured in erythroid differentiation medium (cPIMDM) composed of Iscove Basal Medium (IMDM) (Biochrom) supplemented with 4 mM L-Glutamine (Sigma), 330 μg/ml holo-transferrin (BBI Solutions), 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix), 10 −6 M hydrocortisone (Sigma), 100 ng/ml SCF (R and D Systems), 5 ng/ml IL-3 (R and D Systems), 3 IU/ml Epo (Amgen) and 5% plasma (Octapharma) at 37°C in 5% CO 2 in air, as previously described ( ; ).

    Article Title: AMPK-PERK axis represses oxidative metabolism and enhances apoptotic priming of mitochondria in acute myeloid leukemia.
    Article Snippet: 2015-08-11-DC Primary Human bone marrow CD34+ normal hematopoietic cells StemCell Technologies 70002.2 Chemicals, peptides, and recombinant proteins AMPK activator GSK621 Selleck chemicals S7898, CAS 1346607-05-3 AMPK activator MK8722 Aobious AOB33226, CAS 1394371-71-1 AMPK activator PF739 Aobious AOB33584, CAS 1852452-14-2 ER stress inducer Thapsigargin Sigma-Aldrich T9033, CAS 67526-95-8 AMPK inhibitor Compound C Selleck chemicals S7306, CAS 1219168-18-9 Bcl2 inhibitor Venetoclax Selleck chemicals S8048, CAS 1257044-40-8 6-Bn-ATP-g-S Biolog Life Science Institute B072, CAS 944834-42-8 p- Nitrobenzyl Mesylate Abcam ab138910, CAS 39628-94-9 GST-tagged human recombinant PERK Thermo-Fischer Scientifc PV5106 GST-tagged human recombinant ULK-1 Thermo-Fischer Scientifc PV6430 GST-tagged human recombinant RSK-2 Thermo-Fischer Scientifc PV3323 PUMA2A New England Peptide EQWAREIGAQARRMAADLNA BAD New England Peptide LWAAQRYGRELRRMSDEFEGSFKGL W-HRK New England Peptide WSSAAQLTAARLKALGDELHQ BIM New England Peptide MRPEIWIAQELRRIGDEFNA PUMA New England Peptide EQWAREIGAQLRRMADDLNA NOXAA New England Peptide AELPPEFAAQLRKIGDKVYC (Continued on next page) e2 Cell Reports 38, 110197, January 4, 2022

    Article Title: RNA sequestration in P-bodies sustains myeloid leukemia
    Article Snippet: Primary human bone marrow CD34 + cells (STEMCELL Technologies cat. no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies cat. no 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies cat. no 2690).

    Software:

    Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
    Article Snippet: Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions.

    Article Title: Cancer antigen targets and uses thereof
    Article Snippet: Primary human bone marrow CD34+ cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Flow-cytometric analysis The following antibodies were used to define antigen expression by flow-cytometry: CD82-PE (Biolegend, 342103); CD120b (TNF-RII)-PE (Miltenyi, 130–107-740); EMR2-FITC, -APC (Miltenyi, 130–104-654, 130–104-656); ITGB5-PE (Biolegend, 345203); CCR1-PE, -APC (Miltenyi, 130–100-367, 130–100-358); CD96-APC (Miltenyi, 130–101-031); PTPRJ (CD148)-PE (life technologies, A15799); CD70-PE, -FITC (Biolegend, 355104, 355106); CD85d (ILT4)-PE -APC (Miltenyi, 130–100-567, 130–100-559); LTB4R1-AF700 (Novus Biologicals, FAB099N); CD85h (ILT1)-APC (Miltenyi, 130–100-920); TLR2-APC (Miltenyi, 130–099-020); CR1 (aka CD35)-APC (Miltenyi, 130–099-923); ITGAX (CD11c)-APC (Biolegend, 301613); EMB (abcam, 179801); EMC10 (abcam PA5–25112); LILRB3-PE (Miltenyi, 130–101-662); LILRB4-APC (R&D, FAB24251A); DAGLB (abcam, PA5–26331); P2RY13 (Novus Biologicals, NBP2–37382); LILRA6-APC (Miltenyi, 130–101-665); SLC30A1 (Alomone labs, AZT-011); SLC6A6 (LSBio, LS-C179237); SEMA4A (R&D, FAB4694A); CD123-PE (BD Biosciences, 555644); CLEC12A-PE (Miltenyi, 130–106-482); CD33-APC (Miltenyi, 130–098-864); CD38-BV421 (BD Biosciences, 562444); CD34-PE/Cy7 (Biolegend, 343515); CD45RA-BV640 (Biolegend, 304135); CD90-FITC (BD Biosciences, 555595).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Primary human bone marrow CD34 + cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: RNA sequestration in P-bodies sustains myeloid leukaemia.
    Article Snippet: Srikanth Kodali , Ludovica Proietti , Gemma Valcarcel , Anna V. López-Rubio , Patrizia Pessina , Thomas Eder , Junchao Shi , Annie Jen , Núria Lupión-Garcia , Anne C. Starner, Mason D. Bartels, Yingzhi Cui , Caroline M. Sands , Ainoa Planas-Riverola, Alba Martínez , Talia Velasco-Hernandez , Laureano Tomás-Daza , Bernhard Alber , Gabriele Manhart, Isabella Maria Mayer , Karoline Kollmann , Alessandro Fatica , Pablo Menendez, Evgenia Shishkova, Rachel E. Rau, Biola M. Javierre , Joshua Coon , Qi Chen , Eric L. Van Nostrand , Jose L. Sardina , Florian Grebien & Bruno Di Stefano

    Article Title: Erythrocyte CD55 mediates the internalization of Plasmodium falciparum parasites
    Article Snippet: Bone-marrow-derived primary human CD34 +HSPCs (Stem Cell Technologies) were cultured in erythroid differentiation medium (cPIMDM) composed of Iscove Basal Medium (IMDM) (Biochrom) supplemented with 4 mM L-Glutamine (Sigma), 330 μg/ml holo-transferrin (BBI Solutions), 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix), 10 −6 M hydrocortisone (Sigma), 100 ng/ml SCF (R and D Systems), 5 ng/ml IL-3 (R and D Systems), 3 IU/ml Epo (Amgen) and 5% plasma (Octapharma) at 37°C in 5% CO 2 in air, as previously described ( ; ).

    Article Title: AMPK-PERK axis represses oxidative metabolism and enhances apoptotic priming of mitochondria in acute myeloid leukemia.
    Article Snippet: 2015-08-11-DC Primary Human bone marrow CD34+ normal hematopoietic cells StemCell Technologies 70002.2 Chemicals, peptides, and recombinant proteins AMPK activator GSK621 Selleck chemicals S7898, CAS 1346607-05-3 AMPK activator MK8722 Aobious AOB33226, CAS 1394371-71-1 AMPK activator PF739 Aobious AOB33584, CAS 1852452-14-2 ER stress inducer Thapsigargin Sigma-Aldrich T9033, CAS 67526-95-8 AMPK inhibitor Compound C Selleck chemicals S7306, CAS 1219168-18-9 Bcl2 inhibitor Venetoclax Selleck chemicals S8048, CAS 1257044-40-8 6-Bn-ATP-g-S Biolog Life Science Institute B072, CAS 944834-42-8 p- Nitrobenzyl Mesylate Abcam ab138910, CAS 39628-94-9 GST-tagged human recombinant PERK Thermo-Fischer Scientifc PV5106 GST-tagged human recombinant ULK-1 Thermo-Fischer Scientifc PV6430 GST-tagged human recombinant RSK-2 Thermo-Fischer Scientifc PV3323 PUMA2A New England Peptide EQWAREIGAQARRMAADLNA BAD New England Peptide LWAAQRYGRELRRMSDEFEGSFKGL W-HRK New England Peptide WSSAAQLTAARLKALGDELHQ BIM New England Peptide MRPEIWIAQELRRIGDEFNA PUMA New England Peptide EQWAREIGAQLRRMADDLNA NOXAA New England Peptide AELPPEFAAQLRKIGDKVYC (Continued on next page) e2 Cell Reports 38, 110197, January 4, 2022

    Article Title: RNA sequestration in P-bodies sustains myeloid leukemia
    Article Snippet: Primary human bone marrow CD34 + cells (STEMCELL Technologies cat. no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies cat. no 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies cat. no 2690).

    Sequencing:

    Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
    Article Snippet: Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions.

    Article Title: Cancer antigen targets and uses thereof
    Article Snippet: Primary human bone marrow CD34+ cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Flow-cytometric analysis The following antibodies were used to define antigen expression by flow-cytometry: CD82-PE (Biolegend, 342103); CD120b (TNF-RII)-PE (Miltenyi, 130–107-740); EMR2-FITC, -APC (Miltenyi, 130–104-654, 130–104-656); ITGB5-PE (Biolegend, 345203); CCR1-PE, -APC (Miltenyi, 130–100-367, 130–100-358); CD96-APC (Miltenyi, 130–101-031); PTPRJ (CD148)-PE (life technologies, A15799); CD70-PE, -FITC (Biolegend, 355104, 355106); CD85d (ILT4)-PE -APC (Miltenyi, 130–100-567, 130–100-559); LTB4R1-AF700 (Novus Biologicals, FAB099N); CD85h (ILT1)-APC (Miltenyi, 130–100-920); TLR2-APC (Miltenyi, 130–099-020); CR1 (aka CD35)-APC (Miltenyi, 130–099-923); ITGAX (CD11c)-APC (Biolegend, 301613); EMB (abcam, 179801); EMC10 (abcam PA5–25112); LILRB3-PE (Miltenyi, 130–101-662); LILRB4-APC (R&D, FAB24251A); DAGLB (abcam, PA5–26331); P2RY13 (Novus Biologicals, NBP2–37382); LILRA6-APC (Miltenyi, 130–101-665); SLC30A1 (Alomone labs, AZT-011); SLC6A6 (LSBio, LS-C179237); SEMA4A (R&D, FAB4694A); CD123-PE (BD Biosciences, 555644); CLEC12A-PE (Miltenyi, 130–106-482); CD33-APC (Miltenyi, 130–098-864); CD38-BV421 (BD Biosciences, 562444); CD34-PE/Cy7 (Biolegend, 343515); CD45RA-BV640 (Biolegend, 304135); CD90-FITC (BD Biosciences, 555595).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Primary human bone marrow CD34 + cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: RNA sequestration in P-bodies sustains myeloid leukaemia.
    Article Snippet: Srikanth Kodali , Ludovica Proietti , Gemma Valcarcel , Anna V. López-Rubio , Patrizia Pessina , Thomas Eder , Junchao Shi , Annie Jen , Núria Lupión-Garcia , Anne C. Starner, Mason D. Bartels, Yingzhi Cui , Caroline M. Sands , Ainoa Planas-Riverola, Alba Martínez , Talia Velasco-Hernandez , Laureano Tomás-Daza , Bernhard Alber , Gabriele Manhart, Isabella Maria Mayer , Karoline Kollmann , Alessandro Fatica , Pablo Menendez, Evgenia Shishkova, Rachel E. Rau, Biola M. Javierre , Joshua Coon , Qi Chen , Eric L. Van Nostrand , Jose L. Sardina , Florian Grebien & Bruno Di Stefano

    Article Title: Erythrocyte CD55 mediates the internalization of Plasmodium falciparum parasites
    Article Snippet: Bone-marrow-derived primary human CD34 +HSPCs (Stem Cell Technologies) were cultured in erythroid differentiation medium (cPIMDM) composed of Iscove Basal Medium (IMDM) (Biochrom) supplemented with 4 mM L-Glutamine (Sigma), 330 μg/ml holo-transferrin (BBI Solutions), 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix), 10 −6 M hydrocortisone (Sigma), 100 ng/ml SCF (R and D Systems), 5 ng/ml IL-3 (R and D Systems), 3 IU/ml Epo (Amgen) and 5% plasma (Octapharma) at 37°C in 5% CO 2 in air, as previously described ( ; ).

    Article Title: AMPK-PERK axis represses oxidative metabolism and enhances apoptotic priming of mitochondria in acute myeloid leukemia.
    Article Snippet: 2015-08-11-DC Primary Human bone marrow CD34+ normal hematopoietic cells StemCell Technologies 70002.2 Chemicals, peptides, and recombinant proteins AMPK activator GSK621 Selleck chemicals S7898, CAS 1346607-05-3 AMPK activator MK8722 Aobious AOB33226, CAS 1394371-71-1 AMPK activator PF739 Aobious AOB33584, CAS 1852452-14-2 ER stress inducer Thapsigargin Sigma-Aldrich T9033, CAS 67526-95-8 AMPK inhibitor Compound C Selleck chemicals S7306, CAS 1219168-18-9 Bcl2 inhibitor Venetoclax Selleck chemicals S8048, CAS 1257044-40-8 6-Bn-ATP-g-S Biolog Life Science Institute B072, CAS 944834-42-8 p- Nitrobenzyl Mesylate Abcam ab138910, CAS 39628-94-9 GST-tagged human recombinant PERK Thermo-Fischer Scientifc PV5106 GST-tagged human recombinant ULK-1 Thermo-Fischer Scientifc PV6430 GST-tagged human recombinant RSK-2 Thermo-Fischer Scientifc PV3323 PUMA2A New England Peptide EQWAREIGAQARRMAADLNA BAD New England Peptide LWAAQRYGRELRRMSDEFEGSFKGL W-HRK New England Peptide WSSAAQLTAARLKALGDELHQ BIM New England Peptide MRPEIWIAQELRRIGDEFNA PUMA New England Peptide EQWAREIGAQLRRMADDLNA NOXAA New England Peptide AELPPEFAAQLRKIGDKVYC (Continued on next page) e2 Cell Reports 38, 110197, January 4, 2022

    Article Title: RNA sequestration in P-bodies sustains myeloid leukemia
    Article Snippet: Primary human bone marrow CD34 + cells (STEMCELL Technologies cat. no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies cat. no 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies cat. no 2690).

    Modification:

    Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
    Article Snippet: Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions.

    Article Title: Cancer antigen targets and uses thereof
    Article Snippet: Primary human bone marrow CD34+ cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Flow-cytometric analysis The following antibodies were used to define antigen expression by flow-cytometry: CD82-PE (Biolegend, 342103); CD120b (TNF-RII)-PE (Miltenyi, 130–107-740); EMR2-FITC, -APC (Miltenyi, 130–104-654, 130–104-656); ITGB5-PE (Biolegend, 345203); CCR1-PE, -APC (Miltenyi, 130–100-367, 130–100-358); CD96-APC (Miltenyi, 130–101-031); PTPRJ (CD148)-PE (life technologies, A15799); CD70-PE, -FITC (Biolegend, 355104, 355106); CD85d (ILT4)-PE -APC (Miltenyi, 130–100-567, 130–100-559); LTB4R1-AF700 (Novus Biologicals, FAB099N); CD85h (ILT1)-APC (Miltenyi, 130–100-920); TLR2-APC (Miltenyi, 130–099-020); CR1 (aka CD35)-APC (Miltenyi, 130–099-923); ITGAX (CD11c)-APC (Biolegend, 301613); EMB (abcam, 179801); EMC10 (abcam PA5–25112); LILRB3-PE (Miltenyi, 130–101-662); LILRB4-APC (R&D, FAB24251A); DAGLB (abcam, PA5–26331); P2RY13 (Novus Biologicals, NBP2–37382); LILRA6-APC (Miltenyi, 130–101-665); SLC30A1 (Alomone labs, AZT-011); SLC6A6 (LSBio, LS-C179237); SEMA4A (R&D, FAB4694A); CD123-PE (BD Biosciences, 555644); CLEC12A-PE (Miltenyi, 130–106-482); CD33-APC (Miltenyi, 130–098-864); CD38-BV421 (BD Biosciences, 562444); CD34-PE/Cy7 (Biolegend, 343515); CD45RA-BV640 (Biolegend, 304135); CD90-FITC (BD Biosciences, 555595).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Primary human bone marrow CD34 + cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: RNA sequestration in P-bodies sustains myeloid leukaemia.
    Article Snippet: Srikanth Kodali , Ludovica Proietti , Gemma Valcarcel , Anna V. López-Rubio , Patrizia Pessina , Thomas Eder , Junchao Shi , Annie Jen , Núria Lupión-Garcia , Anne C. Starner, Mason D. Bartels, Yingzhi Cui , Caroline M. Sands , Ainoa Planas-Riverola, Alba Martínez , Talia Velasco-Hernandez , Laureano Tomás-Daza , Bernhard Alber , Gabriele Manhart, Isabella Maria Mayer , Karoline Kollmann , Alessandro Fatica , Pablo Menendez, Evgenia Shishkova, Rachel E. Rau, Biola M. Javierre , Joshua Coon , Qi Chen , Eric L. Van Nostrand , Jose L. Sardina , Florian Grebien & Bruno Di Stefano

    Article Title: Erythrocyte CD55 mediates the internalization of Plasmodium falciparum parasites
    Article Snippet: Bone-marrow-derived primary human CD34 +HSPCs (Stem Cell Technologies) were cultured in erythroid differentiation medium (cPIMDM) composed of Iscove Basal Medium (IMDM) (Biochrom) supplemented with 4 mM L-Glutamine (Sigma), 330 μg/ml holo-transferrin (BBI Solutions), 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix), 10 −6 M hydrocortisone (Sigma), 100 ng/ml SCF (R and D Systems), 5 ng/ml IL-3 (R and D Systems), 3 IU/ml Epo (Amgen) and 5% plasma (Octapharma) at 37°C in 5% CO 2 in air, as previously described ( ; ).

    Article Title: AMPK-PERK axis represses oxidative metabolism and enhances apoptotic priming of mitochondria in acute myeloid leukemia.
    Article Snippet: 2015-08-11-DC Primary Human bone marrow CD34+ normal hematopoietic cells StemCell Technologies 70002.2 Chemicals, peptides, and recombinant proteins AMPK activator GSK621 Selleck chemicals S7898, CAS 1346607-05-3 AMPK activator MK8722 Aobious AOB33226, CAS 1394371-71-1 AMPK activator PF739 Aobious AOB33584, CAS 1852452-14-2 ER stress inducer Thapsigargin Sigma-Aldrich T9033, CAS 67526-95-8 AMPK inhibitor Compound C Selleck chemicals S7306, CAS 1219168-18-9 Bcl2 inhibitor Venetoclax Selleck chemicals S8048, CAS 1257044-40-8 6-Bn-ATP-g-S Biolog Life Science Institute B072, CAS 944834-42-8 p- Nitrobenzyl Mesylate Abcam ab138910, CAS 39628-94-9 GST-tagged human recombinant PERK Thermo-Fischer Scientifc PV5106 GST-tagged human recombinant ULK-1 Thermo-Fischer Scientifc PV6430 GST-tagged human recombinant RSK-2 Thermo-Fischer Scientifc PV3323 PUMA2A New England Peptide EQWAREIGAQARRMAADLNA BAD New England Peptide LWAAQRYGRELRRMSDEFEGSFKGL W-HRK New England Peptide WSSAAQLTAARLKALGDELHQ BIM New England Peptide MRPEIWIAQELRRIGDEFNA PUMA New England Peptide EQWAREIGAQLRRMADDLNA NOXAA New England Peptide AELPPEFAAQLRKIGDKVYC (Continued on next page) e2 Cell Reports 38, 110197, January 4, 2022

    Article Title: RNA sequestration in P-bodies sustains myeloid leukemia
    Article Snippet: Primary human bone marrow CD34 + cells (STEMCELL Technologies cat. no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies cat. no 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies cat. no 2690).

    Synthesized:

    Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
    Article Snippet: Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions.

    Article Title: Cancer antigen targets and uses thereof
    Article Snippet: Primary human bone marrow CD34+ cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Flow-cytometric analysis The following antibodies were used to define antigen expression by flow-cytometry: CD82-PE (Biolegend, 342103); CD120b (TNF-RII)-PE (Miltenyi, 130–107-740); EMR2-FITC, -APC (Miltenyi, 130–104-654, 130–104-656); ITGB5-PE (Biolegend, 345203); CCR1-PE, -APC (Miltenyi, 130–100-367, 130–100-358); CD96-APC (Miltenyi, 130–101-031); PTPRJ (CD148)-PE (life technologies, A15799); CD70-PE, -FITC (Biolegend, 355104, 355106); CD85d (ILT4)-PE -APC (Miltenyi, 130–100-567, 130–100-559); LTB4R1-AF700 (Novus Biologicals, FAB099N); CD85h (ILT1)-APC (Miltenyi, 130–100-920); TLR2-APC (Miltenyi, 130–099-020); CR1 (aka CD35)-APC (Miltenyi, 130–099-923); ITGAX (CD11c)-APC (Biolegend, 301613); EMB (abcam, 179801); EMC10 (abcam PA5–25112); LILRB3-PE (Miltenyi, 130–101-662); LILRB4-APC (R&D, FAB24251A); DAGLB (abcam, PA5–26331); P2RY13 (Novus Biologicals, NBP2–37382); LILRA6-APC (Miltenyi, 130–101-665); SLC30A1 (Alomone labs, AZT-011); SLC6A6 (LSBio, LS-C179237); SEMA4A (R&D, FAB4694A); CD123-PE (BD Biosciences, 555644); CLEC12A-PE (Miltenyi, 130–106-482); CD33-APC (Miltenyi, 130–098-864); CD38-BV421 (BD Biosciences, 562444); CD34-PE/Cy7 (Biolegend, 343515); CD45RA-BV640 (Biolegend, 304135); CD90-FITC (BD Biosciences, 555595).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Primary human bone marrow CD34 + cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: RNA sequestration in P-bodies sustains myeloid leukaemia.
    Article Snippet: Srikanth Kodali , Ludovica Proietti , Gemma Valcarcel , Anna V. López-Rubio , Patrizia Pessina , Thomas Eder , Junchao Shi , Annie Jen , Núria Lupión-Garcia , Anne C. Starner, Mason D. Bartels, Yingzhi Cui , Caroline M. Sands , Ainoa Planas-Riverola, Alba Martínez , Talia Velasco-Hernandez , Laureano Tomás-Daza , Bernhard Alber , Gabriele Manhart, Isabella Maria Mayer , Karoline Kollmann , Alessandro Fatica , Pablo Menendez, Evgenia Shishkova, Rachel E. Rau, Biola M. Javierre , Joshua Coon , Qi Chen , Eric L. Van Nostrand , Jose L. Sardina , Florian Grebien & Bruno Di Stefano

    Article Title: Erythrocyte CD55 mediates the internalization of Plasmodium falciparum parasites
    Article Snippet: Bone-marrow-derived primary human CD34 +HSPCs (Stem Cell Technologies) were cultured in erythroid differentiation medium (cPIMDM) composed of Iscove Basal Medium (IMDM) (Biochrom) supplemented with 4 mM L-Glutamine (Sigma), 330 μg/ml holo-transferrin (BBI Solutions), 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix), 10 −6 M hydrocortisone (Sigma), 100 ng/ml SCF (R and D Systems), 5 ng/ml IL-3 (R and D Systems), 3 IU/ml Epo (Amgen) and 5% plasma (Octapharma) at 37°C in 5% CO 2 in air, as previously described ( ; ).

    Article Title: AMPK-PERK axis represses oxidative metabolism and enhances apoptotic priming of mitochondria in acute myeloid leukemia.
    Article Snippet: 2015-08-11-DC Primary Human bone marrow CD34+ normal hematopoietic cells StemCell Technologies 70002.2 Chemicals, peptides, and recombinant proteins AMPK activator GSK621 Selleck chemicals S7898, CAS 1346607-05-3 AMPK activator MK8722 Aobious AOB33226, CAS 1394371-71-1 AMPK activator PF739 Aobious AOB33584, CAS 1852452-14-2 ER stress inducer Thapsigargin Sigma-Aldrich T9033, CAS 67526-95-8 AMPK inhibitor Compound C Selleck chemicals S7306, CAS 1219168-18-9 Bcl2 inhibitor Venetoclax Selleck chemicals S8048, CAS 1257044-40-8 6-Bn-ATP-g-S Biolog Life Science Institute B072, CAS 944834-42-8 p- Nitrobenzyl Mesylate Abcam ab138910, CAS 39628-94-9 GST-tagged human recombinant PERK Thermo-Fischer Scientifc PV5106 GST-tagged human recombinant ULK-1 Thermo-Fischer Scientifc PV6430 GST-tagged human recombinant RSK-2 Thermo-Fischer Scientifc PV3323 PUMA2A New England Peptide EQWAREIGAQARRMAADLNA BAD New England Peptide LWAAQRYGRELRRMSDEFEGSFKGL W-HRK New England Peptide WSSAAQLTAARLKALGDELHQ BIM New England Peptide MRPEIWIAQELRRIGDEFNA PUMA New England Peptide EQWAREIGAQLRRMADDLNA NOXAA New England Peptide AELPPEFAAQLRKIGDKVYC (Continued on next page) e2 Cell Reports 38, 110197, January 4, 2022

    Article Title: RNA sequestration in P-bodies sustains myeloid leukemia
    Article Snippet: Primary human bone marrow CD34 + cells (STEMCELL Technologies cat. no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies cat. no 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies cat. no 2690).

    Purification:

    Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
    Article Snippet: Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions.

    Article Title: Cancer antigen targets and uses thereof
    Article Snippet: Primary human bone marrow CD34+ cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Flow-cytometric analysis The following antibodies were used to define antigen expression by flow-cytometry: CD82-PE (Biolegend, 342103); CD120b (TNF-RII)-PE (Miltenyi, 130–107-740); EMR2-FITC, -APC (Miltenyi, 130–104-654, 130–104-656); ITGB5-PE (Biolegend, 345203); CCR1-PE, -APC (Miltenyi, 130–100-367, 130–100-358); CD96-APC (Miltenyi, 130–101-031); PTPRJ (CD148)-PE (life technologies, A15799); CD70-PE, -FITC (Biolegend, 355104, 355106); CD85d (ILT4)-PE -APC (Miltenyi, 130–100-567, 130–100-559); LTB4R1-AF700 (Novus Biologicals, FAB099N); CD85h (ILT1)-APC (Miltenyi, 130–100-920); TLR2-APC (Miltenyi, 130–099-020); CR1 (aka CD35)-APC (Miltenyi, 130–099-923); ITGAX (CD11c)-APC (Biolegend, 301613); EMB (abcam, 179801); EMC10 (abcam PA5–25112); LILRB3-PE (Miltenyi, 130–101-662); LILRB4-APC (R&D, FAB24251A); DAGLB (abcam, PA5–26331); P2RY13 (Novus Biologicals, NBP2–37382); LILRA6-APC (Miltenyi, 130–101-665); SLC30A1 (Alomone labs, AZT-011); SLC6A6 (LSBio, LS-C179237); SEMA4A (R&D, FAB4694A); CD123-PE (BD Biosciences, 555644); CLEC12A-PE (Miltenyi, 130–106-482); CD33-APC (Miltenyi, 130–098-864); CD38-BV421 (BD Biosciences, 562444); CD34-PE/Cy7 (Biolegend, 343515); CD45RA-BV640 (Biolegend, 304135); CD90-FITC (BD Biosciences, 555595).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Primary human bone marrow CD34 + cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: RNA sequestration in P-bodies sustains myeloid leukaemia.
    Article Snippet: Srikanth Kodali , Ludovica Proietti , Gemma Valcarcel , Anna V. López-Rubio , Patrizia Pessina , Thomas Eder , Junchao Shi , Annie Jen , Núria Lupión-Garcia , Anne C. Starner, Mason D. Bartels, Yingzhi Cui , Caroline M. Sands , Ainoa Planas-Riverola, Alba Martínez , Talia Velasco-Hernandez , Laureano Tomás-Daza , Bernhard Alber , Gabriele Manhart, Isabella Maria Mayer , Karoline Kollmann , Alessandro Fatica , Pablo Menendez, Evgenia Shishkova, Rachel E. Rau, Biola M. Javierre , Joshua Coon , Qi Chen , Eric L. Van Nostrand , Jose L. Sardina , Florian Grebien & Bruno Di Stefano

    Article Title: Erythrocyte CD55 mediates the internalization of Plasmodium falciparum parasites
    Article Snippet: Bone-marrow-derived primary human CD34 +HSPCs (Stem Cell Technologies) were cultured in erythroid differentiation medium (cPIMDM) composed of Iscove Basal Medium (IMDM) (Biochrom) supplemented with 4 mM L-Glutamine (Sigma), 330 μg/ml holo-transferrin (BBI Solutions), 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix), 10 −6 M hydrocortisone (Sigma), 100 ng/ml SCF (R and D Systems), 5 ng/ml IL-3 (R and D Systems), 3 IU/ml Epo (Amgen) and 5% plasma (Octapharma) at 37°C in 5% CO 2 in air, as previously described ( ; ).

    Article Title: AMPK-PERK axis represses oxidative metabolism and enhances apoptotic priming of mitochondria in acute myeloid leukemia.
    Article Snippet: 2015-08-11-DC Primary Human bone marrow CD34+ normal hematopoietic cells StemCell Technologies 70002.2 Chemicals, peptides, and recombinant proteins AMPK activator GSK621 Selleck chemicals S7898, CAS 1346607-05-3 AMPK activator MK8722 Aobious AOB33226, CAS 1394371-71-1 AMPK activator PF739 Aobious AOB33584, CAS 1852452-14-2 ER stress inducer Thapsigargin Sigma-Aldrich T9033, CAS 67526-95-8 AMPK inhibitor Compound C Selleck chemicals S7306, CAS 1219168-18-9 Bcl2 inhibitor Venetoclax Selleck chemicals S8048, CAS 1257044-40-8 6-Bn-ATP-g-S Biolog Life Science Institute B072, CAS 944834-42-8 p- Nitrobenzyl Mesylate Abcam ab138910, CAS 39628-94-9 GST-tagged human recombinant PERK Thermo-Fischer Scientifc PV5106 GST-tagged human recombinant ULK-1 Thermo-Fischer Scientifc PV6430 GST-tagged human recombinant RSK-2 Thermo-Fischer Scientifc PV3323 PUMA2A New England Peptide EQWAREIGAQARRMAADLNA BAD New England Peptide LWAAQRYGRELRRMSDEFEGSFKGL W-HRK New England Peptide WSSAAQLTAARLKALGDELHQ BIM New England Peptide MRPEIWIAQELRRIGDEFNA PUMA New England Peptide EQWAREIGAQLRRMADDLNA NOXAA New England Peptide AELPPEFAAQLRKIGDKVYC (Continued on next page) e2 Cell Reports 38, 110197, January 4, 2022

    Article Title: RNA sequestration in P-bodies sustains myeloid leukemia
    Article Snippet: Primary human bone marrow CD34 + cells (STEMCELL Technologies cat. no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies cat. no 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies cat. no 2690).

    Invasion Assay:

    Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
    Article Snippet: Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions.

    Article Title: Cancer antigen targets and uses thereof
    Article Snippet: Primary human bone marrow CD34+ cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Flow-cytometric analysis The following antibodies were used to define antigen expression by flow-cytometry: CD82-PE (Biolegend, 342103); CD120b (TNF-RII)-PE (Miltenyi, 130–107-740); EMR2-FITC, -APC (Miltenyi, 130–104-654, 130–104-656); ITGB5-PE (Biolegend, 345203); CCR1-PE, -APC (Miltenyi, 130–100-367, 130–100-358); CD96-APC (Miltenyi, 130–101-031); PTPRJ (CD148)-PE (life technologies, A15799); CD70-PE, -FITC (Biolegend, 355104, 355106); CD85d (ILT4)-PE -APC (Miltenyi, 130–100-567, 130–100-559); LTB4R1-AF700 (Novus Biologicals, FAB099N); CD85h (ILT1)-APC (Miltenyi, 130–100-920); TLR2-APC (Miltenyi, 130–099-020); CR1 (aka CD35)-APC (Miltenyi, 130–099-923); ITGAX (CD11c)-APC (Biolegend, 301613); EMB (abcam, 179801); EMC10 (abcam PA5–25112); LILRB3-PE (Miltenyi, 130–101-662); LILRB4-APC (R&D, FAB24251A); DAGLB (abcam, PA5–26331); P2RY13 (Novus Biologicals, NBP2–37382); LILRA6-APC (Miltenyi, 130–101-665); SLC30A1 (Alomone labs, AZT-011); SLC6A6 (LSBio, LS-C179237); SEMA4A (R&D, FAB4694A); CD123-PE (BD Biosciences, 555644); CLEC12A-PE (Miltenyi, 130–106-482); CD33-APC (Miltenyi, 130–098-864); CD38-BV421 (BD Biosciences, 562444); CD34-PE/Cy7 (Biolegend, 343515); CD45RA-BV640 (Biolegend, 304135); CD90-FITC (BD Biosciences, 555595).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Primary human bone marrow CD34 + cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: RNA sequestration in P-bodies sustains myeloid leukaemia.
    Article Snippet: Srikanth Kodali , Ludovica Proietti , Gemma Valcarcel , Anna V. López-Rubio , Patrizia Pessina , Thomas Eder , Junchao Shi , Annie Jen , Núria Lupión-Garcia , Anne C. Starner, Mason D. Bartels, Yingzhi Cui , Caroline M. Sands , Ainoa Planas-Riverola, Alba Martínez , Talia Velasco-Hernandez , Laureano Tomás-Daza , Bernhard Alber , Gabriele Manhart, Isabella Maria Mayer , Karoline Kollmann , Alessandro Fatica , Pablo Menendez, Evgenia Shishkova, Rachel E. Rau, Biola M. Javierre , Joshua Coon , Qi Chen , Eric L. Van Nostrand , Jose L. Sardina , Florian Grebien & Bruno Di Stefano

    Article Title: Erythrocyte CD55 mediates the internalization of Plasmodium falciparum parasites
    Article Snippet: Bone-marrow-derived primary human CD34 +HSPCs (Stem Cell Technologies) were cultured in erythroid differentiation medium (cPIMDM) composed of Iscove Basal Medium (IMDM) (Biochrom) supplemented with 4 mM L-Glutamine (Sigma), 330 μg/ml holo-transferrin (BBI Solutions), 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix), 10 −6 M hydrocortisone (Sigma), 100 ng/ml SCF (R and D Systems), 5 ng/ml IL-3 (R and D Systems), 3 IU/ml Epo (Amgen) and 5% plasma (Octapharma) at 37°C in 5% CO 2 in air, as previously described ( ; ).

    Article Title: AMPK-PERK axis represses oxidative metabolism and enhances apoptotic priming of mitochondria in acute myeloid leukemia.
    Article Snippet: 2015-08-11-DC Primary Human bone marrow CD34+ normal hematopoietic cells StemCell Technologies 70002.2 Chemicals, peptides, and recombinant proteins AMPK activator GSK621 Selleck chemicals S7898, CAS 1346607-05-3 AMPK activator MK8722 Aobious AOB33226, CAS 1394371-71-1 AMPK activator PF739 Aobious AOB33584, CAS 1852452-14-2 ER stress inducer Thapsigargin Sigma-Aldrich T9033, CAS 67526-95-8 AMPK inhibitor Compound C Selleck chemicals S7306, CAS 1219168-18-9 Bcl2 inhibitor Venetoclax Selleck chemicals S8048, CAS 1257044-40-8 6-Bn-ATP-g-S Biolog Life Science Institute B072, CAS 944834-42-8 p- Nitrobenzyl Mesylate Abcam ab138910, CAS 39628-94-9 GST-tagged human recombinant PERK Thermo-Fischer Scientifc PV5106 GST-tagged human recombinant ULK-1 Thermo-Fischer Scientifc PV6430 GST-tagged human recombinant RSK-2 Thermo-Fischer Scientifc PV3323 PUMA2A New England Peptide EQWAREIGAQARRMAADLNA BAD New England Peptide LWAAQRYGRELRRMSDEFEGSFKGL W-HRK New England Peptide WSSAAQLTAARLKALGDELHQ BIM New England Peptide MRPEIWIAQELRRIGDEFNA PUMA New England Peptide EQWAREIGAQLRRMADDLNA NOXAA New England Peptide AELPPEFAAQLRKIGDKVYC (Continued on next page) e2 Cell Reports 38, 110197, January 4, 2022

    Article Title: RNA sequestration in P-bodies sustains myeloid leukemia
    Article Snippet: Primary human bone marrow CD34 + cells (STEMCELL Technologies cat. no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies cat. no 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies cat. no 2690).

    SYBR Green Assay:

    Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
    Article Snippet: Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions.

    Article Title: Cancer antigen targets and uses thereof
    Article Snippet: Primary human bone marrow CD34+ cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Flow-cytometric analysis The following antibodies were used to define antigen expression by flow-cytometry: CD82-PE (Biolegend, 342103); CD120b (TNF-RII)-PE (Miltenyi, 130–107-740); EMR2-FITC, -APC (Miltenyi, 130–104-654, 130–104-656); ITGB5-PE (Biolegend, 345203); CCR1-PE, -APC (Miltenyi, 130–100-367, 130–100-358); CD96-APC (Miltenyi, 130–101-031); PTPRJ (CD148)-PE (life technologies, A15799); CD70-PE, -FITC (Biolegend, 355104, 355106); CD85d (ILT4)-PE -APC (Miltenyi, 130–100-567, 130–100-559); LTB4R1-AF700 (Novus Biologicals, FAB099N); CD85h (ILT1)-APC (Miltenyi, 130–100-920); TLR2-APC (Miltenyi, 130–099-020); CR1 (aka CD35)-APC (Miltenyi, 130–099-923); ITGAX (CD11c)-APC (Biolegend, 301613); EMB (abcam, 179801); EMC10 (abcam PA5–25112); LILRB3-PE (Miltenyi, 130–101-662); LILRB4-APC (R&D, FAB24251A); DAGLB (abcam, PA5–26331); P2RY13 (Novus Biologicals, NBP2–37382); LILRA6-APC (Miltenyi, 130–101-665); SLC30A1 (Alomone labs, AZT-011); SLC6A6 (LSBio, LS-C179237); SEMA4A (R&D, FAB4694A); CD123-PE (BD Biosciences, 555644); CLEC12A-PE (Miltenyi, 130–106-482); CD33-APC (Miltenyi, 130–098-864); CD38-BV421 (BD Biosciences, 562444); CD34-PE/Cy7 (Biolegend, 343515); CD45RA-BV640 (Biolegend, 304135); CD90-FITC (BD Biosciences, 555595).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Primary human bone marrow CD34 + cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: RNA sequestration in P-bodies sustains myeloid leukaemia.
    Article Snippet: Srikanth Kodali , Ludovica Proietti , Gemma Valcarcel , Anna V. López-Rubio , Patrizia Pessina , Thomas Eder , Junchao Shi , Annie Jen , Núria Lupión-Garcia , Anne C. Starner, Mason D. Bartels, Yingzhi Cui , Caroline M. Sands , Ainoa Planas-Riverola, Alba Martínez , Talia Velasco-Hernandez , Laureano Tomás-Daza , Bernhard Alber , Gabriele Manhart, Isabella Maria Mayer , Karoline Kollmann , Alessandro Fatica , Pablo Menendez, Evgenia Shishkova, Rachel E. Rau, Biola M. Javierre , Joshua Coon , Qi Chen , Eric L. Van Nostrand , Jose L. Sardina , Florian Grebien & Bruno Di Stefano

    Article Title: Erythrocyte CD55 mediates the internalization of Plasmodium falciparum parasites
    Article Snippet: Bone-marrow-derived primary human CD34 +HSPCs (Stem Cell Technologies) were cultured in erythroid differentiation medium (cPIMDM) composed of Iscove Basal Medium (IMDM) (Biochrom) supplemented with 4 mM L-Glutamine (Sigma), 330 μg/ml holo-transferrin (BBI Solutions), 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix), 10 −6 M hydrocortisone (Sigma), 100 ng/ml SCF (R and D Systems), 5 ng/ml IL-3 (R and D Systems), 3 IU/ml Epo (Amgen) and 5% plasma (Octapharma) at 37°C in 5% CO 2 in air, as previously described ( ; ).

    Article Title: AMPK-PERK axis represses oxidative metabolism and enhances apoptotic priming of mitochondria in acute myeloid leukemia.
    Article Snippet: 2015-08-11-DC Primary Human bone marrow CD34+ normal hematopoietic cells StemCell Technologies 70002.2 Chemicals, peptides, and recombinant proteins AMPK activator GSK621 Selleck chemicals S7898, CAS 1346607-05-3 AMPK activator MK8722 Aobious AOB33226, CAS 1394371-71-1 AMPK activator PF739 Aobious AOB33584, CAS 1852452-14-2 ER stress inducer Thapsigargin Sigma-Aldrich T9033, CAS 67526-95-8 AMPK inhibitor Compound C Selleck chemicals S7306, CAS 1219168-18-9 Bcl2 inhibitor Venetoclax Selleck chemicals S8048, CAS 1257044-40-8 6-Bn-ATP-g-S Biolog Life Science Institute B072, CAS 944834-42-8 p- Nitrobenzyl Mesylate Abcam ab138910, CAS 39628-94-9 GST-tagged human recombinant PERK Thermo-Fischer Scientifc PV5106 GST-tagged human recombinant ULK-1 Thermo-Fischer Scientifc PV6430 GST-tagged human recombinant RSK-2 Thermo-Fischer Scientifc PV3323 PUMA2A New England Peptide EQWAREIGAQARRMAADLNA BAD New England Peptide LWAAQRYGRELRRMSDEFEGSFKGL W-HRK New England Peptide WSSAAQLTAARLKALGDELHQ BIM New England Peptide MRPEIWIAQELRRIGDEFNA PUMA New England Peptide EQWAREIGAQLRRMADDLNA NOXAA New England Peptide AELPPEFAAQLRKIGDKVYC (Continued on next page) e2 Cell Reports 38, 110197, January 4, 2022

    Article Title: RNA sequestration in P-bodies sustains myeloid leukemia
    Article Snippet: Primary human bone marrow CD34 + cells (STEMCELL Technologies cat. no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies cat. no 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies cat. no 2690).

    Staining:

    Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
    Article Snippet: Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions.

    Article Title: Cancer antigen targets and uses thereof
    Article Snippet: Primary human bone marrow CD34+ cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Flow-cytometric analysis The following antibodies were used to define antigen expression by flow-cytometry: CD82-PE (Biolegend, 342103); CD120b (TNF-RII)-PE (Miltenyi, 130–107-740); EMR2-FITC, -APC (Miltenyi, 130–104-654, 130–104-656); ITGB5-PE (Biolegend, 345203); CCR1-PE, -APC (Miltenyi, 130–100-367, 130–100-358); CD96-APC (Miltenyi, 130–101-031); PTPRJ (CD148)-PE (life technologies, A15799); CD70-PE, -FITC (Biolegend, 355104, 355106); CD85d (ILT4)-PE -APC (Miltenyi, 130–100-567, 130–100-559); LTB4R1-AF700 (Novus Biologicals, FAB099N); CD85h (ILT1)-APC (Miltenyi, 130–100-920); TLR2-APC (Miltenyi, 130–099-020); CR1 (aka CD35)-APC (Miltenyi, 130–099-923); ITGAX (CD11c)-APC (Biolegend, 301613); EMB (abcam, 179801); EMC10 (abcam PA5–25112); LILRB3-PE (Miltenyi, 130–101-662); LILRB4-APC (R&D, FAB24251A); DAGLB (abcam, PA5–26331); P2RY13 (Novus Biologicals, NBP2–37382); LILRA6-APC (Miltenyi, 130–101-665); SLC30A1 (Alomone labs, AZT-011); SLC6A6 (LSBio, LS-C179237); SEMA4A (R&D, FAB4694A); CD123-PE (BD Biosciences, 555644); CLEC12A-PE (Miltenyi, 130–106-482); CD33-APC (Miltenyi, 130–098-864); CD38-BV421 (BD Biosciences, 562444); CD34-PE/Cy7 (Biolegend, 343515); CD45RA-BV640 (Biolegend, 304135); CD90-FITC (BD Biosciences, 555595).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Primary human bone marrow CD34 + cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: RNA sequestration in P-bodies sustains myeloid leukaemia.
    Article Snippet: Srikanth Kodali , Ludovica Proietti , Gemma Valcarcel , Anna V. López-Rubio , Patrizia Pessina , Thomas Eder , Junchao Shi , Annie Jen , Núria Lupión-Garcia , Anne C. Starner, Mason D. Bartels, Yingzhi Cui , Caroline M. Sands , Ainoa Planas-Riverola, Alba Martínez , Talia Velasco-Hernandez , Laureano Tomás-Daza , Bernhard Alber , Gabriele Manhart, Isabella Maria Mayer , Karoline Kollmann , Alessandro Fatica , Pablo Menendez, Evgenia Shishkova, Rachel E. Rau, Biola M. Javierre , Joshua Coon , Qi Chen , Eric L. Van Nostrand , Jose L. Sardina , Florian Grebien & Bruno Di Stefano

    Article Title: Erythrocyte CD55 mediates the internalization of Plasmodium falciparum parasites
    Article Snippet: Bone-marrow-derived primary human CD34 +HSPCs (Stem Cell Technologies) were cultured in erythroid differentiation medium (cPIMDM) composed of Iscove Basal Medium (IMDM) (Biochrom) supplemented with 4 mM L-Glutamine (Sigma), 330 μg/ml holo-transferrin (BBI Solutions), 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix), 10 −6 M hydrocortisone (Sigma), 100 ng/ml SCF (R and D Systems), 5 ng/ml IL-3 (R and D Systems), 3 IU/ml Epo (Amgen) and 5% plasma (Octapharma) at 37°C in 5% CO 2 in air, as previously described ( ; ).

    Article Title: AMPK-PERK axis represses oxidative metabolism and enhances apoptotic priming of mitochondria in acute myeloid leukemia.
    Article Snippet: 2015-08-11-DC Primary Human bone marrow CD34+ normal hematopoietic cells StemCell Technologies 70002.2 Chemicals, peptides, and recombinant proteins AMPK activator GSK621 Selleck chemicals S7898, CAS 1346607-05-3 AMPK activator MK8722 Aobious AOB33226, CAS 1394371-71-1 AMPK activator PF739 Aobious AOB33584, CAS 1852452-14-2 ER stress inducer Thapsigargin Sigma-Aldrich T9033, CAS 67526-95-8 AMPK inhibitor Compound C Selleck chemicals S7306, CAS 1219168-18-9 Bcl2 inhibitor Venetoclax Selleck chemicals S8048, CAS 1257044-40-8 6-Bn-ATP-g-S Biolog Life Science Institute B072, CAS 944834-42-8 p- Nitrobenzyl Mesylate Abcam ab138910, CAS 39628-94-9 GST-tagged human recombinant PERK Thermo-Fischer Scientifc PV5106 GST-tagged human recombinant ULK-1 Thermo-Fischer Scientifc PV6430 GST-tagged human recombinant RSK-2 Thermo-Fischer Scientifc PV3323 PUMA2A New England Peptide EQWAREIGAQARRMAADLNA BAD New England Peptide LWAAQRYGRELRRMSDEFEGSFKGL W-HRK New England Peptide WSSAAQLTAARLKALGDELHQ BIM New England Peptide MRPEIWIAQELRRIGDEFNA PUMA New England Peptide EQWAREIGAQLRRMADDLNA NOXAA New England Peptide AELPPEFAAQLRKIGDKVYC (Continued on next page) e2 Cell Reports 38, 110197, January 4, 2022

    Article Title: RNA sequestration in P-bodies sustains myeloid leukemia
    Article Snippet: Primary human bone marrow CD34 + cells (STEMCELL Technologies cat. no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies cat. no 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies cat. no 2690).

    Expressing:

    Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
    Article Snippet: Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions.

    Article Title: Cancer antigen targets and uses thereof
    Article Snippet: Primary human bone marrow CD34+ cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Flow-cytometric analysis The following antibodies were used to define antigen expression by flow-cytometry: CD82-PE (Biolegend, 342103); CD120b (TNF-RII)-PE (Miltenyi, 130–107-740); EMR2-FITC, -APC (Miltenyi, 130–104-654, 130–104-656); ITGB5-PE (Biolegend, 345203); CCR1-PE, -APC (Miltenyi, 130–100-367, 130–100-358); CD96-APC (Miltenyi, 130–101-031); PTPRJ (CD148)-PE (life technologies, A15799); CD70-PE, -FITC (Biolegend, 355104, 355106); CD85d (ILT4)-PE -APC (Miltenyi, 130–100-567, 130–100-559); LTB4R1-AF700 (Novus Biologicals, FAB099N); CD85h (ILT1)-APC (Miltenyi, 130–100-920); TLR2-APC (Miltenyi, 130–099-020); CR1 (aka CD35)-APC (Miltenyi, 130–099-923); ITGAX (CD11c)-APC (Biolegend, 301613); EMB (abcam, 179801); EMC10 (abcam PA5–25112); LILRB3-PE (Miltenyi, 130–101-662); LILRB4-APC (R&D, FAB24251A); DAGLB (abcam, PA5–26331); P2RY13 (Novus Biologicals, NBP2–37382); LILRA6-APC (Miltenyi, 130–101-665); SLC30A1 (Alomone labs, AZT-011); SLC6A6 (LSBio, LS-C179237); SEMA4A (R&D, FAB4694A); CD123-PE (BD Biosciences, 555644); CLEC12A-PE (Miltenyi, 130–106-482); CD33-APC (Miltenyi, 130–098-864); CD38-BV421 (BD Biosciences, 562444); CD34-PE/Cy7 (Biolegend, 343515); CD45RA-BV640 (Biolegend, 304135); CD90-FITC (BD Biosciences, 555595).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Primary human bone marrow CD34 + cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: RNA sequestration in P-bodies sustains myeloid leukaemia.
    Article Snippet: Srikanth Kodali , Ludovica Proietti , Gemma Valcarcel , Anna V. López-Rubio , Patrizia Pessina , Thomas Eder , Junchao Shi , Annie Jen , Núria Lupión-Garcia , Anne C. Starner, Mason D. Bartels, Yingzhi Cui , Caroline M. Sands , Ainoa Planas-Riverola, Alba Martínez , Talia Velasco-Hernandez , Laureano Tomás-Daza , Bernhard Alber , Gabriele Manhart, Isabella Maria Mayer , Karoline Kollmann , Alessandro Fatica , Pablo Menendez, Evgenia Shishkova, Rachel E. Rau, Biola M. Javierre , Joshua Coon , Qi Chen , Eric L. Van Nostrand , Jose L. Sardina , Florian Grebien & Bruno Di Stefano

    Article Title: Erythrocyte CD55 mediates the internalization of Plasmodium falciparum parasites
    Article Snippet: Bone-marrow-derived primary human CD34 +HSPCs (Stem Cell Technologies) were cultured in erythroid differentiation medium (cPIMDM) composed of Iscove Basal Medium (IMDM) (Biochrom) supplemented with 4 mM L-Glutamine (Sigma), 330 μg/ml holo-transferrin (BBI Solutions), 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix), 10 −6 M hydrocortisone (Sigma), 100 ng/ml SCF (R and D Systems), 5 ng/ml IL-3 (R and D Systems), 3 IU/ml Epo (Amgen) and 5% plasma (Octapharma) at 37°C in 5% CO 2 in air, as previously described ( ; ).

    Article Title: AMPK-PERK axis represses oxidative metabolism and enhances apoptotic priming of mitochondria in acute myeloid leukemia.
    Article Snippet: 2015-08-11-DC Primary Human bone marrow CD34+ normal hematopoietic cells StemCell Technologies 70002.2 Chemicals, peptides, and recombinant proteins AMPK activator GSK621 Selleck chemicals S7898, CAS 1346607-05-3 AMPK activator MK8722 Aobious AOB33226, CAS 1394371-71-1 AMPK activator PF739 Aobious AOB33584, CAS 1852452-14-2 ER stress inducer Thapsigargin Sigma-Aldrich T9033, CAS 67526-95-8 AMPK inhibitor Compound C Selleck chemicals S7306, CAS 1219168-18-9 Bcl2 inhibitor Venetoclax Selleck chemicals S8048, CAS 1257044-40-8 6-Bn-ATP-g-S Biolog Life Science Institute B072, CAS 944834-42-8 p- Nitrobenzyl Mesylate Abcam ab138910, CAS 39628-94-9 GST-tagged human recombinant PERK Thermo-Fischer Scientifc PV5106 GST-tagged human recombinant ULK-1 Thermo-Fischer Scientifc PV6430 GST-tagged human recombinant RSK-2 Thermo-Fischer Scientifc PV3323 PUMA2A New England Peptide EQWAREIGAQARRMAADLNA BAD New England Peptide LWAAQRYGRELRRMSDEFEGSFKGL W-HRK New England Peptide WSSAAQLTAARLKALGDELHQ BIM New England Peptide MRPEIWIAQELRRIGDEFNA PUMA New England Peptide EQWAREIGAQLRRMADDLNA NOXAA New England Peptide AELPPEFAAQLRKIGDKVYC (Continued on next page) e2 Cell Reports 38, 110197, January 4, 2022

    Article Title: RNA sequestration in P-bodies sustains myeloid leukemia
    Article Snippet: Primary human bone marrow CD34 + cells (STEMCELL Technologies cat. no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies cat. no 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies cat. no 2690).

    Standard Deviation:

    Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
    Article Snippet: Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions.

    Article Title: Cancer antigen targets and uses thereof
    Article Snippet: Primary human bone marrow CD34+ cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Flow-cytometric analysis The following antibodies were used to define antigen expression by flow-cytometry: CD82-PE (Biolegend, 342103); CD120b (TNF-RII)-PE (Miltenyi, 130–107-740); EMR2-FITC, -APC (Miltenyi, 130–104-654, 130–104-656); ITGB5-PE (Biolegend, 345203); CCR1-PE, -APC (Miltenyi, 130–100-367, 130–100-358); CD96-APC (Miltenyi, 130–101-031); PTPRJ (CD148)-PE (life technologies, A15799); CD70-PE, -FITC (Biolegend, 355104, 355106); CD85d (ILT4)-PE -APC (Miltenyi, 130–100-567, 130–100-559); LTB4R1-AF700 (Novus Biologicals, FAB099N); CD85h (ILT1)-APC (Miltenyi, 130–100-920); TLR2-APC (Miltenyi, 130–099-020); CR1 (aka CD35)-APC (Miltenyi, 130–099-923); ITGAX (CD11c)-APC (Biolegend, 301613); EMB (abcam, 179801); EMC10 (abcam PA5–25112); LILRB3-PE (Miltenyi, 130–101-662); LILRB4-APC (R&D, FAB24251A); DAGLB (abcam, PA5–26331); P2RY13 (Novus Biologicals, NBP2–37382); LILRA6-APC (Miltenyi, 130–101-665); SLC30A1 (Alomone labs, AZT-011); SLC6A6 (LSBio, LS-C179237); SEMA4A (R&D, FAB4694A); CD123-PE (BD Biosciences, 555644); CLEC12A-PE (Miltenyi, 130–106-482); CD33-APC (Miltenyi, 130–098-864); CD38-BV421 (BD Biosciences, 562444); CD34-PE/Cy7 (Biolegend, 343515); CD45RA-BV640 (Biolegend, 304135); CD90-FITC (BD Biosciences, 555595).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Primary human bone marrow CD34 + cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: RNA sequestration in P-bodies sustains myeloid leukaemia.
    Article Snippet: Srikanth Kodali , Ludovica Proietti , Gemma Valcarcel , Anna V. López-Rubio , Patrizia Pessina , Thomas Eder , Junchao Shi , Annie Jen , Núria Lupión-Garcia , Anne C. Starner, Mason D. Bartels, Yingzhi Cui , Caroline M. Sands , Ainoa Planas-Riverola, Alba Martínez , Talia Velasco-Hernandez , Laureano Tomás-Daza , Bernhard Alber , Gabriele Manhart, Isabella Maria Mayer , Karoline Kollmann , Alessandro Fatica , Pablo Menendez, Evgenia Shishkova, Rachel E. Rau, Biola M. Javierre , Joshua Coon , Qi Chen , Eric L. Van Nostrand , Jose L. Sardina , Florian Grebien & Bruno Di Stefano

    Article Title: Erythrocyte CD55 mediates the internalization of Plasmodium falciparum parasites
    Article Snippet: Bone-marrow-derived primary human CD34 +HSPCs (Stem Cell Technologies) were cultured in erythroid differentiation medium (cPIMDM) composed of Iscove Basal Medium (IMDM) (Biochrom) supplemented with 4 mM L-Glutamine (Sigma), 330 μg/ml holo-transferrin (BBI Solutions), 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix), 10 −6 M hydrocortisone (Sigma), 100 ng/ml SCF (R and D Systems), 5 ng/ml IL-3 (R and D Systems), 3 IU/ml Epo (Amgen) and 5% plasma (Octapharma) at 37°C in 5% CO 2 in air, as previously described ( ; ).

    Article Title: AMPK-PERK axis represses oxidative metabolism and enhances apoptotic priming of mitochondria in acute myeloid leukemia.
    Article Snippet: 2015-08-11-DC Primary Human bone marrow CD34+ normal hematopoietic cells StemCell Technologies 70002.2 Chemicals, peptides, and recombinant proteins AMPK activator GSK621 Selleck chemicals S7898, CAS 1346607-05-3 AMPK activator MK8722 Aobious AOB33226, CAS 1394371-71-1 AMPK activator PF739 Aobious AOB33584, CAS 1852452-14-2 ER stress inducer Thapsigargin Sigma-Aldrich T9033, CAS 67526-95-8 AMPK inhibitor Compound C Selleck chemicals S7306, CAS 1219168-18-9 Bcl2 inhibitor Venetoclax Selleck chemicals S8048, CAS 1257044-40-8 6-Bn-ATP-g-S Biolog Life Science Institute B072, CAS 944834-42-8 p- Nitrobenzyl Mesylate Abcam ab138910, CAS 39628-94-9 GST-tagged human recombinant PERK Thermo-Fischer Scientifc PV5106 GST-tagged human recombinant ULK-1 Thermo-Fischer Scientifc PV6430 GST-tagged human recombinant RSK-2 Thermo-Fischer Scientifc PV3323 PUMA2A New England Peptide EQWAREIGAQARRMAADLNA BAD New England Peptide LWAAQRYGRELRRMSDEFEGSFKGL W-HRK New England Peptide WSSAAQLTAARLKALGDELHQ BIM New England Peptide MRPEIWIAQELRRIGDEFNA PUMA New England Peptide EQWAREIGAQLRRMADDLNA NOXAA New England Peptide AELPPEFAAQLRKIGDKVYC (Continued on next page) e2 Cell Reports 38, 110197, January 4, 2022

    Article Title: RNA sequestration in P-bodies sustains myeloid leukemia
    Article Snippet: Primary human bone marrow CD34 + cells (STEMCELL Technologies cat. no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies cat. no 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies cat. no 2690).

    Drug discovery:

    Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
    Article Snippet: Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions.

    Article Title: Cancer antigen targets and uses thereof
    Article Snippet: Primary human bone marrow CD34+ cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Flow-cytometric analysis The following antibodies were used to define antigen expression by flow-cytometry: CD82-PE (Biolegend, 342103); CD120b (TNF-RII)-PE (Miltenyi, 130–107-740); EMR2-FITC, -APC (Miltenyi, 130–104-654, 130–104-656); ITGB5-PE (Biolegend, 345203); CCR1-PE, -APC (Miltenyi, 130–100-367, 130–100-358); CD96-APC (Miltenyi, 130–101-031); PTPRJ (CD148)-PE (life technologies, A15799); CD70-PE, -FITC (Biolegend, 355104, 355106); CD85d (ILT4)-PE -APC (Miltenyi, 130–100-567, 130–100-559); LTB4R1-AF700 (Novus Biologicals, FAB099N); CD85h (ILT1)-APC (Miltenyi, 130–100-920); TLR2-APC (Miltenyi, 130–099-020); CR1 (aka CD35)-APC (Miltenyi, 130–099-923); ITGAX (CD11c)-APC (Biolegend, 301613); EMB (abcam, 179801); EMC10 (abcam PA5–25112); LILRB3-PE (Miltenyi, 130–101-662); LILRB4-APC (R&D, FAB24251A); DAGLB (abcam, PA5–26331); P2RY13 (Novus Biologicals, NBP2–37382); LILRA6-APC (Miltenyi, 130–101-665); SLC30A1 (Alomone labs, AZT-011); SLC6A6 (LSBio, LS-C179237); SEMA4A (R&D, FAB4694A); CD123-PE (BD Biosciences, 555644); CLEC12A-PE (Miltenyi, 130–106-482); CD33-APC (Miltenyi, 130–098-864); CD38-BV421 (BD Biosciences, 562444); CD34-PE/Cy7 (Biolegend, 343515); CD45RA-BV640 (Biolegend, 304135); CD90-FITC (BD Biosciences, 555595).

    Article Title: Integrating proteomics and transcriptomics for systematic combinatorial chimeric antigen receptor therapy of AML
    Article Snippet: Primary human bone marrow CD34 + cells were purchased from Stem Cell Technologies (70002.2, 70002.3).

    Article Title: RNA sequestration in P-bodies sustains myeloid leukaemia.
    Article Snippet: Srikanth Kodali , Ludovica Proietti , Gemma Valcarcel , Anna V. López-Rubio , Patrizia Pessina , Thomas Eder , Junchao Shi , Annie Jen , Núria Lupión-Garcia , Anne C. Starner, Mason D. Bartels, Yingzhi Cui , Caroline M. Sands , Ainoa Planas-Riverola, Alba Martínez , Talia Velasco-Hernandez , Laureano Tomás-Daza , Bernhard Alber , Gabriele Manhart, Isabella Maria Mayer , Karoline Kollmann , Alessandro Fatica , Pablo Menendez, Evgenia Shishkova, Rachel E. Rau, Biola M. Javierre , Joshua Coon , Qi Chen , Eric L. Van Nostrand , Jose L. Sardina , Florian Grebien & Bruno Di Stefano

    Article Title: Erythrocyte CD55 mediates the internalization of Plasmodium falciparum parasites
    Article Snippet: Bone-marrow-derived primary human CD34 +HSPCs (Stem Cell Technologies) were cultured in erythroid differentiation medium (cPIMDM) composed of Iscove Basal Medium (IMDM) (Biochrom) supplemented with 4 mM L-Glutamine (Sigma), 330 μg/ml holo-transferrin (BBI Solutions), 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix), 10 −6 M hydrocortisone (Sigma), 100 ng/ml SCF (R and D Systems), 5 ng/ml IL-3 (R and D Systems), 3 IU/ml Epo (Amgen) and 5% plasma (Octapharma) at 37°C in 5% CO 2 in air, as previously described ( ; ).

    Article Title: AMPK-PERK axis represses oxidative metabolism and enhances apoptotic priming of mitochondria in acute myeloid leukemia.
    Article Snippet: 2015-08-11-DC Primary Human bone marrow CD34+ normal hematopoietic cells StemCell Technologies 70002.2 Chemicals, peptides, and recombinant proteins AMPK activator GSK621 Selleck chemicals S7898, CAS 1346607-05-3 AMPK activator MK8722 Aobious AOB33226, CAS 1394371-71-1 AMPK activator PF739 Aobious AOB33584, CAS 1852452-14-2 ER stress inducer Thapsigargin Sigma-Aldrich T9033, CAS 67526-95-8 AMPK inhibitor Compound C Selleck chemicals S7306, CAS 1219168-18-9 Bcl2 inhibitor Venetoclax Selleck chemicals S8048, CAS 1257044-40-8 6-Bn-ATP-g-S Biolog Life Science Institute B072, CAS 944834-42-8 p- Nitrobenzyl Mesylate Abcam ab138910, CAS 39628-94-9 GST-tagged human recombinant PERK Thermo-Fischer Scientifc PV5106 GST-tagged human recombinant ULK-1 Thermo-Fischer Scientifc PV6430 GST-tagged human recombinant RSK-2 Thermo-Fischer Scientifc PV3323 PUMA2A New England Peptide EQWAREIGAQARRMAADLNA BAD New England Peptide LWAAQRYGRELRRMSDEFEGSFKGL W-HRK New England Peptide WSSAAQLTAARLKALGDELHQ BIM New England Peptide MRPEIWIAQELRRIGDEFNA PUMA New England Peptide EQWAREIGAQLRRMADDLNA NOXAA New England Peptide AELPPEFAAQLRKIGDKVYC (Continued on next page) e2 Cell Reports 38, 110197, January 4, 2022

    Article Title: RNA sequestration in P-bodies sustains myeloid leukemia
    Article Snippet: Primary human bone marrow CD34 + cells (STEMCELL Technologies cat. no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies cat. no 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies cat. no 2690).



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    ( A ) Clustering of the DEGs during transdifferentiation between dCas9-DNMT3A CTRL and IL1RN edited cells. ( n = 2 biologically independent replicates, FDR < 0.05). TC1 to TC4, transdifferentiation clusters. Representative genes for each cluster are annotated. ( B ) Gene Ontology Biological Processes (GO:BP) enrichment analysis for the genes associated with clusters in (A). The top five most significant terms for each cluster are plotted. Significant terms ( q value < 0.05) are highlighted with a black stroke. ( C ) Mean fluorescence intensity (MFI) of macrophage-lineage surface markers in CTRL and edited iMacs. ( D ) Representative histograms showing signal intensity for selected cell surface markers in CTRL and edited iMacs. ( E ) Phagocytic capacity evaluation [by fluorescence-activated cell sorting (FACS)] for CTRL and edited iMacs. Left, histogram showing the uptake of blue fluorescent beads. Right, blue beads MFI quantification. Unpaired two-tailed Student’s t test, n = 3, means ± SEM, (*** P < 0.001). ( F ) Up, schematic of the IL1RN shRNAs experiments during transdifferentiation. Bottom, MFI quantification of myeloid markers in shLuc and shIL1RN iMacs. Two-way ANOVA with Dunnett’s post hoc test, n = 3, means ± SEM, (**** P < 0.0001). ( G ) Phagocytic capacity evaluation (by FACS) for shLuc and shIL1RN iMacs. Left, histogram showing the uptake of blue fluorescent beads. Right, blue beads MFI quantification. Two-way ANOVA with Dunnett’s post hoc test, n = 3, means ± SEM, (**** P < 0.0001). ( H ) Left, schematic of the colony-forming assay performed in shLuc and shIL1RN bone marrow–derived human CD34 + cells; CFU, colony forming unit. Right, total number of CFUs obtained from shLuc and shIL1RN_1/_2 CD34 + cells. n = 3 biologically independent samples per group, means ± SEM. ( I ) Comparison of different types of CFUs obtained from shLuc and shIL1RN_1/_2 CD34 + cells. Two-way ANOVA with Dunnett’s post hoc test, n = 3, means ± SEM,(* P < 0.05; ** P < 0.01; **** P < 0.0001).

    Journal: Science Advances

    Article Title: Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing

    doi: 10.1126/sciadv.adt1644

    Figure Lengend Snippet: ( A ) Clustering of the DEGs during transdifferentiation between dCas9-DNMT3A CTRL and IL1RN edited cells. ( n = 2 biologically independent replicates, FDR < 0.05). TC1 to TC4, transdifferentiation clusters. Representative genes for each cluster are annotated. ( B ) Gene Ontology Biological Processes (GO:BP) enrichment analysis for the genes associated with clusters in (A). The top five most significant terms for each cluster are plotted. Significant terms ( q value < 0.05) are highlighted with a black stroke. ( C ) Mean fluorescence intensity (MFI) of macrophage-lineage surface markers in CTRL and edited iMacs. ( D ) Representative histograms showing signal intensity for selected cell surface markers in CTRL and edited iMacs. ( E ) Phagocytic capacity evaluation [by fluorescence-activated cell sorting (FACS)] for CTRL and edited iMacs. Left, histogram showing the uptake of blue fluorescent beads. Right, blue beads MFI quantification. Unpaired two-tailed Student’s t test, n = 3, means ± SEM, (*** P < 0.001). ( F ) Up, schematic of the IL1RN shRNAs experiments during transdifferentiation. Bottom, MFI quantification of myeloid markers in shLuc and shIL1RN iMacs. Two-way ANOVA with Dunnett’s post hoc test, n = 3, means ± SEM, (**** P < 0.0001). ( G ) Phagocytic capacity evaluation (by FACS) for shLuc and shIL1RN iMacs. Left, histogram showing the uptake of blue fluorescent beads. Right, blue beads MFI quantification. Two-way ANOVA with Dunnett’s post hoc test, n = 3, means ± SEM, (**** P < 0.0001). ( H ) Left, schematic of the colony-forming assay performed in shLuc and shIL1RN bone marrow–derived human CD34 + cells; CFU, colony forming unit. Right, total number of CFUs obtained from shLuc and shIL1RN_1/_2 CD34 + cells. n = 3 biologically independent samples per group, means ± SEM. ( I ) Comparison of different types of CFUs obtained from shLuc and shIL1RN_1/_2 CD34 + cells. Two-way ANOVA with Dunnett’s post hoc test, n = 3, means ± SEM,(* P < 0.05; ** P < 0.01; **** P < 0.0001).

    Article Snippet: Primary human bone-marrow CD34 + cells (STEMCELL Technologies, catalog no. 70002.3) were cultured in StemSpan SFEM (STEMCELL Technologies, catalog no. 9600) supplemented with StemSpan TM CC100 (STEMCELL Technologies, catalog no. 2690). shLuc or IL1RN shRNA_1/2–expressing viruses were centrifuged onto RetroNectin-coated plates prepared according to manufacturer’s instructions.

    Techniques: Fluorescence, FACS, Two Tailed Test, Derivative Assay, Comparison